微生物蛋白与转基因作物蛋白等同性比较

Comparison of Equivalence for Micro-Produced Protein with Transgenic Protein

转基因作物的风险性评估一般需要大量的转基因蛋白,但是人们很难从转基因作物中表达纯化出足够量的蛋白。然而,人们可以通过微生物过表达系统获得大量的纯化蛋白,如果可以用微生物蛋白来代替转基因蛋白进行风险性研究,就可以解决这一难题,但是微生物蛋白与转基因蛋白必须在生化特性与功能特性上具有等同性。简要介绍一套典型的比较微生物蛋白和转基因蛋白等同性的方法,为利用微生物蛋白进行转基因作物的风险性评估提供参考。

The studies for risk assessment of transgenic crops require amounts of transgenic protein,but it is extremely difficult to prepare the required amount of purified protein from transgenic plants.Nevertheless,ample protein of high purity may be produced by over-expressing the protein in microbes.Using microbial proteins in a study for risk assessment can solve this problem,but the microbial proteins must be equivalent with transgenic plant proteins.In this article,we describe a typical set of methods used to compare the equivalence for micro-protein with transgenic protein,which for the purposes of providing reference for the risk assessment using micro-proteins instead of trans-proteins.