野生金龙胆草遗传多样性的RAPD分析及总黄酮成分在局群间的分布特征

Genetic Diversity of Wild Populations of Conyza blinii Lévl. and Extraction of Total Flavonoids

探索来自18个采集地的金龙胆草RAPD遗传性分析及总黄酮成分在局群间的分布特征。采用筛选的10个随机引物,用NTSYSpc2.1软件计算材料间的Jaccard遗传相似系数(GS),并建立各种间的亲缘关系树形图,并利用紫外分光光度法测试各样品中总黄酮的含量。10个引物共扩出71个DNA片段,多态性DNA片段68个,占总扩增数的97.14%。18个金龙胆草种源可明显聚为2大类。总黄酮含量以攀枝花市范围的样品含量最高。不同种源金龙胆草间具有丰富的遗传多样性,总黄酮含量分布却与遗传聚类的分类有差异,提示今后在金龙胆草选育和道地药材研究上应该从多方面进行考虑。

The genetic relationship of Conyza bliniiLévl.was analyzed in molecular level and the distribution characteristics of total flavonoid constituents.The RAPD method was applied to the study onConyza bliniiLévl.from different germplasms by use of 10 random primers about 10 bp,and the parameters were calculated by NTSYSpc2.1 and the relationship was constructed based on UPGMA.Use UV spectrophotometry to test total flavonoids in each sample.10 primers screened out from 100 primers were used for RAPD amplification.A total of 71 bands were generated,of which 68 bands were polymorphism bands.According to RAPD analysis,18 germplasms ofConyza bliniiLévl.were classified into two categories.The genetic diversity of the 18 species inConyza bliniiLévl.is high and the total flavinoids was related of geographic distribution.