摘要
为提高灰褐链霉菌抗菌活性物质的产量,采用单因素优化的方法对灰褐链霉菌SC-4所产生的抗菌活性物质的发酵条件进行优化,结果显示最佳的发酵条件为:温度37℃、起始pH7、接种量6%、转速180 r/min和发酵时间为5 d,通过优化,链霉菌SC-4产生的抗菌物质产量提高,抑菌圈直径能够达到20 mm。为获得单一的该抗菌活性物质,并最终明确其结构,采用硫酸铵分级沉淀、半制备型高效液相色谱(HPLC)等方法对菌株SC-4所产生抗菌物质进行分离纯化,最终通过HPLC获得了4个活性峰,其中1号峰活性最大,4号峰活性很小。这些结果为链霉菌SC-4产生的抗菌活性物质的结构鉴定及新型抗生素的开发提供了基础资料。
A Streptomyce SC-4 with high antibacterial activation was screened, it was identified as Streptomyces griseofuscus. For enhance the antibacterialthe substances antibacterial substance fermentation conditions were optimized using single factor method, and the results showed that the optimal conditions were temperature 37 ℃, initial pH7, inoculum 6%, rotation speed 180 r/min and fermentation 5 days. To separate and purify the antibacterial substance, the method of ammonium sulfate fractionation and HPLC were used. Finally, four active peaks were obtained with HPLC, the peak 1 is high and peak 4 is low. These maybe have some means for structure identification and new antibiotics development.
出处
《食品科技》
CAS
北大核心
2013年第9期189-192,共4页
Food Science and Technology
基金
江苏省高校自然科学基金项目(11KJD550002)
江苏省科技厅科技支撑计划项目(BE2011644)
徐州市科技计划项目(XF11C056)
关键词
链霉菌
抑菌活性物质
分离纯化
发酵条件优化
Streptomyce
antibacterial substance
separation and purification
optimization fermentation conditions