实时荧光PCR快速筛选食品中鸭源性成分

Detection for duck-derived ingredients in foods with real-time polymerase chain reaction method

基于鸭线粒体细胞色素Cyt b基因建立了肉制品中的鸭源性成分检测的real-time PCR方法,并对模拟样本和市售样本进行了检测分析,检出限低至1%,适用于实验室的鸭源性成分的鉴定分析。

A rapidly and specific real-time PCR assay,bansed on the amplification of the Cyt b gene, had been developed for the detection of duck-derived ingredients in food. Simulation samples and commercially processed food testing showed that it was possible to detect 1% duck spiked in other species and it was a potentially reliable and suitable technique in routine food analysis for detection of duck-derived ingredients in food.