摘要
以荷叶离褶伞茵丝体为材料,利DEAE—Sepharose F.F.色谱和sephadexG-100色谱技术,分离获得新的LMLLML。根据凝胶过滤色谱和SDS—PAGE电泳结果,确定LML是中性糖含量为10.36%、分子量约为48.2ku单亚基糖蛋白。凝血活性检测结果表明,LML对兔血红细胞具有凝聚活性,这种活性在LML浓度为500μg/mL、温度为20~70℃、pH3~10的条件下稳定;其凝血活性可被葡萄糖(最小浓度为25mmol/L)和麦芽糖(最小浓度为12.5mmol/L)所抑制;当LML浓度≥250μg/mL时,其凝血活性不受Ca^2+、Mg^2+和Zn^2+的影响。
One novel lectin LML was purified from mycelium of Lyophyllum decastes using ion exchange chromatography and gel filtration. Based on the result of gel filtration and SDS-PAGE,LML was single subunit glycoprotein with a molecular mass of 48.2ku and having 10.36% neutral sugar. The hemagglutinating activity test showed that LML showed hemagglutinating activity on rabbit red blood cells. Its activity was stable in the pH range of 3-10,temperature range of 20-70℃, The hemagglutinating activity of LML were inhibited by glucose (25mmol/L) and maltose (12.5mmol/L) ,and were unaffected by Ca2+, Mg2+ and Zn2+ at LML concentrition≥ 250μg/m L.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第18期114-118,共5页
Science and Technology of Food Industry
基金
甘肃省科技支撑项目(090HKCG068)
关键词
祁连山
荷叶离褶伞
菌丝体
LML
凝血活性
Qilian mountain
Lyophyllum decastes
mycelium
LML
hemagg lutinating activity