海蜇糖蛋白及其糖肽的体外免疫活性

Effects of Glycoprotein and Glycopeptides from Jellyfish on Immune Function of Mice in vitro

研究海蜇糖蛋白及其糖肽体外免疫活性。通过小鼠脾淋巴细胞增殖和转化实验,比较海蜇糖蛋白及其糖肽在不同培养时间、不同质量浓度条件下对小鼠体外免疫的影响。结果显示:不同培养时间、不同质量浓度的海蜇糖蛋白及糖肽均能不同程度地提高小鼠脾淋巴细胞的增殖和转化能力,且表现出一定剂量关系。糖肽Ⅰ组在提高小鼠脾淋巴细胞的增殖和转化能力方面显著优于其他几个组分,当糖肽Ⅰ组质量浓度为50 g/mL,培养60h对脾淋巴细胞的增殖和转化作用最强。

The immunoregulatory activity of glycoprotein and glycopeptides from jellyfish was explored through cell proliferation and cell transformation of mouse spleen lymphocytes and comparatively evaluated under various cultivation times and concentrations. Jellyfish glycoprotein and glycopeptides revealed significant increase in the proliferation and transformation of spleen lymphocytes in a dose-dependent manner. The results demonstrated that glycopeptides had better immunoregulatory activity in vitro when spleen lymphocytes were cultivated for 60 hours and the concentration of glycopeptides was 50 μg/mL.