天然虾青素在卤虫体内的代谢积累研究

A Preliminary Investigation of Astaxanthin Metabolism and Accumulation in Artemia

目的:探索冰凉花中的虾青素在卤虫体内代谢积累情况。方法:在实验室内对卤虫卵进行孵化,养殖为成虫进行饥饿处理后饲喂冰凉花,分别在喂食后0.5、1、2、3、4、5h内取定量卤虫进行萃取,使用高效液相色谱(HPLC)分析。结果:卤虫进食冰凉花后,游离态虾青素的含量开始增加,并且在3h达到峰值,与此同时,卤虫体内的虾青素酯逐渐消失。结论:冰凉花中的虾青素酯可以在卤虫体内转化为游离态虾青素并进行积累。

This study was aimed to investigate the in vivo metabolism and accumulation of astaxanthin from Adonis aestivalis in Artemia （brine shrimps）. A total of 1400 48-h fasted adult brine shrimps were randomly divided into two groups （700 brine shrimps per group）： control and experimental groups. Shrimps were fed on dried powder of Adonis aestivalis flower in experimental group and each group was extracted at 0.5, 1, 2, 3, 4 h and 5 h for C18-HPLC analysis. Results showed that free astaxanthin started to accumulate in shrimps following feeding of Adonis aestivalis flower and reached the maximum in 3 h. In addition, astaxanthin ethers in brine shrimps gradually disappeared. In conclusion, administration of Adonis aestivalis flower powder to brine shrimps led to an increase in free astaxanthin. The astaxanthin eithers from Adonis aestivalis flower were metabolically transformed into free astaxanthin and accumulated in vivo.