普鲁托品固体脂质纳米粒的制备及抗炎效果

Preparation and Anti-inflammatory Activity of Protopine Solid Lipid Nanoparticles

采用热熔分散法制备不同处方的普鲁托品固体脂质纳米粒用于透皮给药。结果以三硬脂酸甘油酯为脂质材料、大豆磷脂为乳化剂的制品粒径最小[(126.2±1.7)nm]、包封率最高(92.7%)。体外透皮试验表明,该制品12 h累积渗透率为78.5%,明显高于溶液组(32.4%)。并且,该制品对Freund's完全佐剂所致的佐剂性关节炎大鼠有较强的疗效,抗炎活性与扶他林组相当。

The protopine solid lipid nanoparticles with different formulations for transdermal delivery were prepared by melt dispersion technique. The results showed that the particle size of the product prepared with glyceryl tristearate as lipid material and soybean phospholipid as emulsifier was the smallest E （126.2±1.7）nm] and its encapsulation efficiency was the highest （92.7 %）. The in vitro cumulative permeation amount at 12 h of the product was 78.5 %, which was significantly higher than that of its solution （32.4%）. In addition, it had a stronger effect on adjuvant arthritis rats caused by Freund＇s complete adjuvant. The anti-inflammatory activity of the product was similar with the Voltaren group.