超声-微波协同辅助萃取茶皂素

Ultrasonic and microwave-assisted extraction of tea saponin

采用超声-微波协同辅助萃取脱脂油茶籽粕中的茶皂素。在单因素试验的基础上,固定超声波功率50 W,萃取次数2次,以乙醇体积分数、料液比、微波功率和萃取时间为影响因素,茶皂素得率为指标,正交试验进行优化,最终确定的茶皂素萃取工艺条件为:乙醇体积分数50%,料液比1∶10,微波功率600 W,超声波功率50 W,萃取时间150 s,萃取次数2次。在此条件下,茶皂素得率为(17.43±0.13)%,纯度为67.12%。

The ultrasonic and microwave - assisted extraction of tea saponin from defatted oil - tea camel- lia seed meal was studied. Fixing ultrasonic power 50 W and extraction times twice, the influences of eth- anol volume fraction, ratio of solid to liquid, microwave power and extraction time on tea saponin yield were investigated by othorgonal experiment on the basis of single factor experiment. The results showed that the optimal extraction conditions of tea saponin were as follows： ethanol volume fraction 50%, ratio of solid to liquid 1： 10, microwave power 600 W, ultrasonic power 50 W, extraction time 150 s, extraction times twice. Under these conditions, the tea saponin yield was （17.43 ±0.13）% and the purity was 67.12%.