一种通用型颤振珠磨式微生物快速裂解系统的设计与实现

Design and implementation of a universal bead-milling and vibrating lytic system for rapid lysis of microbes

目的开发一种结构简单、操作方便、运行平稳、成本较低的通用型颤振珠磨式微生物快速裂解系统,解决临床检验和诊断对微生物快速裂解的迫切需求。方法设计分析了传动机构和控制电路,然后加工装配制作系统样机。选择金黄色葡萄球菌作为裂解对象,通过平板培养统计裂解率,并探索裂解率与浓度、裂解时间和电机转速之间的关系。结果相同裂解条件下,低浓度的金黄色葡萄球菌的裂解率高于高浓度;电机转速相同时,不同浓度的金黄色葡萄球菌的裂解率均随裂解时间的增加而提高,呈"S"型变化;随着电机转速的增加,金黄色葡萄球菌的最终裂解率逐渐提高。结论裂解释放DNA的定量检测结果证明所设计系统能够有效裂解微生物样本,其裂解效能优于实验室常用裂解方式。

Objective To develop a universal bead-milling and vibrating lytic system that is simple-structured,userfriendly,smooth-running and inexpensive to meet the demands of rapid lysis of microbes for clinical examination and diagnosis.Methods The experimental prototype machine was made after the design and analysis of the transmission mechanism as well as control circuit.With Staphylococcus aureus as test object,this paper calculated its lysis rate by plate cultivation method,and then explored how the lysis rate changed with concentration,processing time and motor speed.Results Compared with S.aureus of higher concentrations,the lower one had a higher lysis rate.With the extension of processing time,the lysis rate increased,which presented an ＂ S＂ type curve.The final lysis rate increased gradually with motor speed.Conclusion The DNA extracted from S.aureus is detected quantitatively,the results of which showed that the designed system could effectively lyse microbe samples in a comparatively short time.