摘要
目的探讨龙血竭乙醇提取物(Ethanolic extract of Resina Draconis,RDEE)促进创面愈合的疗效。方法将48只SD大鼠随机分为对照组、湿润烧伤膏组(MEBO组)和龙血竭乙醇提取物组(RDEE组)。测量和计算伤后第3、7、11和15天创面面积,计算创面愈合率和愈合时间;采用HE、Masson染色和CD31免疫组织化学染色,观察创面肉芽组织结构改变、胶原分布,并计算微血管密度(Microvessel density,MVD);采用荧光定量PCR和Western Blot,检测创面肉芽组织中VEGF表达的变化。结果 RDEE组创面愈合时间明显比对照组短(P<0.05),MEBO组和RDEE组之间无显著性差异;RDEE组、MEBO组创面愈合率和伤后第3、7、11天的MVD、VEGF表达量均高于对照组,差异显著(P<0.05);RDEE组创面结构和胶原排列方式均优于对照组。结论龙血竭提取物具有促进创面愈合的作用,值得临床推广应用。
Objective To investigate the effects of ethanolic extract of Resina Draconis (RDEE) in animal wound healing. Methods Forty-eight SD rats were randomly divided into three groups: control group, MEBO group (treated with MEBO) and RDEE group (treated with RDEE). Wound healing rates and healing time were calculated 3, 7, 11 and 15 days after treatment, and tissues were harvested at the same time for histological, immunohistochemical analysis and MVD calculation. The expression of VEGF was determined by real-time PCR and western blot. Results Wound healing time in RDEE group was shorter than in control group (P〈0.05). There was no difference of would healing time between RDEE group and MEBO group. Wound healing rates, MVD number (3, 7, 11 days after treatment) and the expression of VEGF were significantly higher in RDEE group and MEBO group than in control group (P〈0.05). Histological results showed more well-organized bands of collagen, more fibrob-lasts and less inflammatory cells in RDEE group compared with control group. Conclusion The extract from Resina Draconis possesses wound healing activity, and is worthy of clinical application.
出处
《组织工程与重建外科杂志》
2013年第4期199-203,共5页
Journal of Tissue Engineering and Reconstructive Surgery
基金
上海市科学技术委员会中医现代化项目(09DZ1970400)
关键词
龙血竭提取物
创面愈合
微血管密度
Resina Draconis extract Wound healing Microvessel density