量子点为载体转染survivin siRNA至人舌鳞癌Tca8113细胞的研究

The Study of Quantum Dots as Vectors in Targeted Survivin Gene siRNA Delivery of Tca8113 Tongue Cancer Cells

目的靶向survivin基因的siRNA通过量子点为载体转染至人舌鳞癌Tca8113细胞中,研究其对survivin基因表达的影响,为量子点在肿瘤的基因治疗方面提供实验依据。方法 Survivin siRNA通过量子点转染至人舌鳞癌Tca8113细胞中,同时设立LIPOFECTTAMINE2000脂质体为转染载体的对照组以及未转染的空白组,采用实时定量RT-PCR检测各组Tca8113细胞中survivin mRNA表达的改变情况。结果实验组用50pmolQD转染100pmol survivin siRNA入Tca8113细胞48h后,检测到survivin siRNA mRNA表达量相对于空白组下降64%,QD与siRNA的使用比例为1∶2;对照组中用100pmol lipofectamine2000脂质体转染100pmol survivin siRNA入Tca8113细胞48h后,检测到survivin siRNA mRNA表达量相对于空白组下降90%,QD与siRNA的使用比例为1∶1。结论量子点为载体转染survivin siRNA能够有效的抑制Tca8113细胞survivin mRNA的表达,量子点有望作为RNA干扰技术的新型载体。

Objective To study the change of survivin gene transcription levels influenced by quantom dots, which are used as a vector to deliver survivin siRNA to human tongue cancer Tca8113 cells, and provide experimental basis for tumor gene therapy of quantum dots. Methods Quantom dots can deliver survivin siRNA to human tongue cancer Tca8113 cells. In order to detect the change of survivin gene transcription levels of Tca8113 cells, two groups, the control group, which used LIPOFECTTAMINE 2000 liposomes as transfection vector and the blank group which was not transfected, were established. And each group＇s change of survivin gene transcription levels of Tca8113 cells was detected by the real-time quantitative RT-PCR. Results The control group used 50 pmolQD to deliver 100pmol survivin siRNA into Tca8113 cells, 48h later, survivin siRNA mRNA transcription level decreased to 36% compared with the blank group （the ratio of QD and siRNA used was 1：2）; the control group used 100 pmol lipofectamine2000 Liposome to deliver 100pmol survivin siRNA into Tca8113 cells, 48h later, survivin siRNA mRNA transcription level decreased to 10% compared with the blank group （the ratio of lipofectamine2000 and siRNA used was 1：1） Conclusion Quantum dots can be vectors to transfect survivin siRNA into Tca8113 human tongue cancer cells and effectively inhibit the transcription level of survivn mRNA of Tca8113 cells, so quantum dots would be a new vector of RNA interference technology.