加标回收实验及质控图在初级水产品药残检测中的应用

Applications of standard addition test and quality control chart in drug residues detection of primary aquatic products

介绍了加标回收实验和质控图作为质量控制的方法,并根据2012年实验室液相色谱-质谱联用法检测初级水产品中孔雀石绿和硝基呋喃类代谢物的加标回收率进行统计分析,其中质控图用Origin软件进行绘制,数据关联性用SPSS软件进行分析。结果显示,加标回收率质控图能够有效地对实验结果进行监控,并且有效地保证数据质量;引起本实验室孔雀石绿和硝基呋喃类代谢物加标回收率变化的主要因素是随机误差;隐色孔雀石绿与显色孔雀石绿加标回收率之间具有较弱的相关性,另外隐色孔雀石绿的平均回收率略高于显色孔雀石绿;4种硝基呋喃类代谢物中,AOZ和AMOZ、SEM的加标回收率之间有良好的相关性,其他代谢物之间相关性较差,另外AOZ的平均加标回收率最高,SEM的最低。

The quality control methods of standard addition test and quality control charts were introduced. According to the standard addition detection of malachite green and nitrofuran metabolites in primary aquatic products by LC/MS, the standard addition recoveries were analyzed. The quality control charts were drew by Origin, and the correlation of standard addition recoveries was analyzed by SPSS. The results indicated that the quality control charts could monitor the test results effectively, and they could ensure the detection results were accurate. The main reason which caused the variation of standard addition recycles was random error. The recoveries correlation between color malachite green and leuco malachite green was weak. The average standard addition recycles of leuco malachite green was higher than that of color malachite green. In four kinds of nitrofuran metabolites, the recoveries correlation between AOZ and AMOZ or SEM was better than others. The average standard addition recycles of AOZ was maximum, and the average standard addition recycles of SEM was minimum.