病毒性心肌炎细胞因子及T细胞亚群的变化

Analysis of Cytokines and T-Cell Subsets in Patients with Viral Myocarditis

目的探讨白介素6(IL-6)、白介素8(IL-8)、肿瘤坏死因子(TNF),T细胞亚群(CD3、CD4、CD8)与病毒性心肌炎(viral myocarditis,VMC)的相关性。方法选择42例心肌炎患者(VMC组)和42名健康人(对照组),采用双抗体夹心酶联免疫吸附法(ELISA)测定血清IL-6、IL-8,采用放射免疫分析法测定TNF,采用ABC免疫酶染色法测定T细胞亚群。比较两组的各个检测指标。结果 VMC组IL-6、IL-8、TNF显著高于对照组,T细胞亚群中CD4、CD8明显低于对照组,CD4/CD8显著高于对照组。结论 IL-6、IL-8、TNF参与了发病,一系列细胞因子介导的免疫、炎性反应、免疫调节功能的失衡在病毒性心肌炎的发生、发展中起重要作用。

Objective To investigate the correlation of interleukin 6（IL-6）,interleukin 8（IL-8）,tumor necrosis factor（TNF）,T cell subsets（CD3,CD4,CD8） and viral myocarditis（VMC）.Methods Forty-two patients with VMC（VMC group） and 42 healthy people（control group） were selected in this study.Double antibody sandwich enzyme-linked immunosorbent assay（ELISA） was used to detect the serum IL-6 and IL-8 level,radio immunoassay（RIA） was used to detect the TNF,and the ABC immunohistochemical staining method was used to detect the T cell subsets levels.The detection indices of two groups were compared.Results In the VMC group,IL-6,IL-8 and TNF-α were significantly higher,CD4 and CD8 of T cell subsets were significantly lower,the ratio of CD4/CD8 was significantly higher.Conclusions IL-6,IL-8 and TNF-α involve in the disease;a series of cytokine-mediated immune and inflammatory response play an important role in the development of VMC.