摘要
目的研究肿瘤坏死因子样微弱凋亡诱导因子(TWEAK)对体外培养骨关节炎(OA)软骨细胞的作用,同时探讨炎性细胞因子白细胞介素-1β(IL-1β)和肿瘤坏死因子α(TNF-α)与TWEAK之间的关系。方法体外培养OA患者原代软骨细胞,分别用TWEAK(75ng/mL),TWEAK(75ng/mL)和IL-1β(5ng/mL),TWEAK(75ng/mL)和TNF-α(5ng/mL)作用细胞48h,MTT检测细胞因子对软骨细胞的增殖作用影响;ELISA检测细胞培养上清液中MMP-13、IL-6和Fas的分泌表达。结果 MTT结果表明细胞因子刺激软骨细胞后对细胞增殖无明显影响。TWEAK和IL-1β组诱导MMP-13表达增加(P<0.05);TWEAK和IL-1β组,TWEAK和TNF-α组均诱导IL-6表达增加(P<0.05)。3组Fas分泌表达差异无统计学意义(P>0.05)。结论 IL-1β与TWEAK,TNF-α与TWEAK分别诱导OA软骨细胞MMP-13和IL-6表达增加,可能参与关节软骨损伤和OA的病程发展。
Objective To investigate the role of TWEAK on the OA chondrocytes,and to discuss the relationship between IL-1β and TWEAK,TNF-α and TWEAK.Methods Chondrocytes from OA patients were cultured in vitro,stimulated by TWEAK(75 ng/mL),TWEAK(75 ng/mL) with IL-1β(5 ng/mL),TWEAK(75 ng/mL) with TNFα(5 ng/mL) for 48 h.The cells proliferation rate was analyzed by MTT.ELISA was used to detect the concentration of MMP-13,IL-6 and Fas in supernatant.Results The chondrocytes proliferation was not influenced by these cytokines.The expression of MMP-13 could be induced by TWEAK with IL-1β stimulation(P0.05).IL-6 expression induced by TWEAK with IL-1β or TWEAK with TNF-α were higher than the other groups(P0.05).There was no significance change in the expression of Fas.Conclusion TWEAK with IL-1β,TWEAK with TNF-α could induce the OA chondrocytes to synthesize MMP-13 and IL-6,which might affect pathological process of OA.
出处
《检验医学与临床》
CAS
2013年第16期2065-2066,2068,共3页
Laboratory Medicine and Clinic
基金
国家自然科学基金(30972749)
四川省科技厅应用基础项目(2008JY0013)