强直性脊柱炎患者外周血T淋巴细胞亚群的表达

The expression of T lymphocyte subgroup in peripheral blood of ankylosing spondylitis patients

目的探讨强直性脊柱炎(AS)患者外周血CD4+CD45RA+、CD4+CD45RO+、CD8+CD45RA+和CD8+CD45RO+T淋巴细胞亚群的表达及与免疫功能的关系。方法用流式细胞仪检测55例AS患者(分AS病情活动组30例、稳定组25例)CD4+、CD8+细胞CD45RO、CD45RA的表达,并与健康对照组进行比较。结果 AS患者活动期CD4+CD45RO+、CD8+CD45RO+T淋巴细胞表达明显高于健康对照组(P<0.01),CD4+CD45RA+、CD8+CD45RA+明显对低于健康对照组(P<0.01);稳定期CD4+CD45RO+、CD8+CD45RO+与活动期比较明显降低(P<0.05),但仍高于健康对照组(P<0.05);CD4+CD45RA+、CD8+CD45RA+与活动期相比明显升高(P<0.05),但仍低于健康对照组(P<0.05)。结论 CD4+CD45RO+、CD8+CD45RO+和CD4+CD45RA+、CD8+CD45RA+T淋巴细胞的异常表达是导致强直性脊柱炎患者机体免疫功能失去平衡的重要原因。

Objective To discuss on the peripheral blood CD4＋CD45RA＋,CD4＋CD45RO＋,CD8＋CD45RA＋ and CD8＋CD45RO＋T lymphocyte subgroup expression and immune function in patients with ankylosing spondylitis（AS）.Methods To detect the expression of CD45RO and CD45RA in CD4＋ and CD8＋ T lymphocyte by flow cytometry in patients and compared with the healthy control group.Results Compare with healthy control group,the expressions of CD4＋CD45RO＋and CD8＋CD45RO＋T lymphocyte in patients active stage was significantly increased（P0.01）,but the expression of CD4＋CD45RA＋ and CD8＋CD45RA＋ decreased obviosly（P0.01）.The expressions of CD4＋CD45RO＋and CD8＋CD45RO＋T lymphocyte in patients stable stage was significantly lower than that in patients active stage（P0.05）,but which was still higher than that in healthy control group（P0.05）.The expression of CD4＋CD45RA＋ and CD8＋CD45RA＋ T lymphocyte in patients stable stage was significantly higher than that in patients active stage（P0.05）,but which was still lower than that in healthy control group（P0.05）.Conclusion The abnormal expresions of CD4＋CD45RO＋、CD8＋CD45RO＋ and CD4＋CD45RA＋、CD8＋CD45RA＋T lymphocyte was one of the immportant reason for patients inmmune fuction disbalance with ankylosing spondylitis.