胡杨离体快繁及生根技术研究

Research on Rapid Propagation and Rooting of in Vitro Culture in Populus Euphratica

采用MS为基本培养基,通过加入不同浓度的的生长调节剂,对胡杨的离体增殖与生根培养基进行筛选,结果表明:胡杨离体增殖培养的最适培养基配方为MS+6-BA1.0 mg/L+NAA0.5 mg/L,增殖率达87%;最佳生根培养基为1/2MS+IBA1.5 mg/L,生根率达到了97%,并将生根的胡杨苗进行移栽,成活率达24%。

Rapid propagation and rooting in vitro culture of Populus euphratica were studied,which used MS as the basic culture medium with different concentrations of plant-growing regulator. The results showed that the optimum medium for rapid propagation was MS＋6-BA 1.0 mg/L＋NAA 0.5 mg/L,the propagation rate was 87%.The optimum medium for rooting was 1/2 MS＋IBA1.5 mg/L ,the rooting rate could reach up to 97%. Meanwhile survival rate of transplanting the rooting populus euphratica seedlings reached 24%.