正交试验法优化罗布麻叶中总黄酮的闪式提取工艺

The Optimization of Flash-type Extractional Process of Total Flavonoids in Apocynum Venetum Leaves by Orthogonal Experiment

[目的]优化闪式提取器提取罗布麻叶中总黄酮成分的工艺条件。[方法]在单因素试验的基础上,运用L9(34)正交试验优选罗布麻叶总黄酮的闪式提取工艺。[结果]闪式提取法提取罗布麻叶中总黄酮的最佳工艺为:乙醇浓度55%,料液比1∶50(W/V,g/ml,下同),提取时间50 s,提取次数2次;在此条件下,最高提取率为4.24%。验证性试验用最佳工艺条件提取5次,RSD为1.07%。[结论]该工艺稳定可行,闪式提取高效、快速,不破坏成分,有推广价值。

[Objective] To explore the best process of flash-type extractor for extracting total flavonoids in Apocynum venetum leaves.[Method] Using L9 （34） orthogonal experimental method to optimize the flash-type extractional process of total flavonoids in Apocynum venetum leaves by single factor and orthogonal experiment.[Result] The optimal extraction technology is：ethanol concentration 55％,ratio of solid to liquid 1：50,extraction time 50 s,2 times,the highest extraction rate is 4.24％.The optimum technique conditions for verification test is extracting for 5 times,RSD is 1.07％.[Conclusion] The process is stable and feasible,suitable for promotion,and the flash-type extraction is efficient,fast and no damage for the components.