加味圣愈汤对创伤性脑损伤大鼠的神经保护作用及其机制探讨

Neuroprotective effects of modified Shengyu Decoction on rats with traumatic brain injury

目的观察加味圣愈汤对创伤性脑损伤(TBI)大鼠的神经保护作用,并探讨其作用机制。方法将98只SD大鼠随机分为A组22只、B组22只、C组54只。B、C组采用改良的Feeney法制备TBI模型,A组仅开骨窗不打击。造模后6 h,C组使用不同剂量的加味圣愈汤(0.5 mL/200 g,16只;1.0 mL/200 g,22只;2.0 mL/200 g,16只)灌胃治疗2次/d。A、B组仅给予等体积的生理盐水灌胃。对伤后大鼠进行神经行为学观察,并采用干湿法测量脑含水量,尼氏染色光镜下观察海马细胞形态,测量脑组织损伤体积,ELISA法测定脑外伤后72 h脑组织中的TNF-α、IL-1β、IL-6、IL-10。结果与B组比较,C组神经行为测试评分明显提高,脑含水量降低,海马神经细胞损伤减轻,TNF-α、IL-1β水平升高及IL-10水平下降均受到抑制(P均<0.05),而脑组织损伤体积和IL-6的表达未见明显改变(P均>0.05)。结论加味圣愈汤治疗脑外伤对脑组织有保护作用,其机制可能与调节脑组织中炎症相关因子的表达,从而减轻炎症反应有关。

Objective To observe the neuroprotective effects of modified Shengyu Decoction on rats with traumatic brain injury （TBI） and to investigate the underlying mechanism. Methods Ninety-eight rats were randomly divided into 3 groups： group A （n =22）, group B （n =22） and group C （n =54）. In group B and C, the modified Feeney＇s method was used to establish TBI model, and only bone window was opened in rats of group A. After modeling for 6 h, rats in group C were intragastrically treated with 0.5, 1.0 and 2.0 mL/200 g modified Shengyu Decoction （ n = 16, 22 and 16, respectively; twice a day）, while rats in group A and B were treated with the same volume of normal saline. The neurological functions of rats after injury were observed. The brain water content was measured with wet-dry weight method. The morphological change of neuron in hippocampal area and cerebral contusion volume were observed under optical microscope through Nissl staining. The contents of TNF-α, IL-1β, IL-6, and IL-10 in the brain tissue were detected by ELISA method. Results After the modified Shengyu Decoction treatment, compared with that of group B, recovery of the neurological functions was promoted, the brain water content was decreased, the hippocampal injury was alleviated, and the contents of TNF-α and IL-1β were significantly increased, and IL-10 was decreased （ all P 〈 0.05） while the contusion volume of brain tissue and the expression of IL-6 were not significantly changed （ all P 〉 0.05 ）. Conclusion Modified Shengyu Decoction has a neuroprotective effect on the brain of rats after traumatic brain injuries. The mechanism may be related to a regulatory effect on the expression of inflammatory cytokines in the brain tissue, which alleviates the cerebral inflammatory response.