HPLC法测定痹痛贴中蛇床子素与二氢欧山芹醇当归酸酯的含量

Determination of Osthole and Columbianadin in Bi-Tong Paste by HPLC

目的：建立痹痛贴中蛇床子素与二氢欧山芹醇当归酸酯的含量HPLC分析方法。方法：采用ChromstarTMCl8（4．6x250toni，5μm）色谱柱，以乙腈-水（60：40）为流动相，流速1．0mL／min，检测波长330nm，柱温30℃。结果：蛇床子素进样量在0．149—1．192p,g线性关系良好，平均回收率为97．17％，RSD=0．24％（n=6）；二氢欧山芹醇当归酸酯进样量在0．054—0．432gg线性关系良好，平均回收率为95．98％，RSD=0．77％（n=6）。结论：该方法简单、重复性好，可用于痹痛贴的质量控制。

Objective： To establish I-IPLC method for determination osthole and Columbianadin in ＂Bi-tong paste＂ . Methods： The samples were separated at 30~C on a ChromstarTMCls （4. 6×2.50mm, 5 μm） column eluted with acetonitrile-water （60：40） as the mobile phase. Flow rate was set at 1.0mI_/min and the detection wavelength was set at 330 nm. Results： Osthole showed a good linear relation in the range of 0. 149 - 1. 192μg The average recovery was 97. 17% with RSD of 0.24% （ n=6） . Columbianadin showed a good linear relation in the range of 0. 054 ～ 0. 432μg The average recovery was 95. 98% with RSD of 0. 77% （ n = 6）. Conclusion： The method is accurate, simple and with good reproducibility. It can be used for the quality control of ＂Bi-tong paste＂.