食品中亚硝酸盐现场快速定量检测系统的研制

Development of Field Rapid Quantitative Detection System for Nitrite in Food

研究了一种用于现场检测食品质量产品的"检测系统"——食品中亚硝酸盐现场快速定量检测系统,该检测系统由主机、试剂盒、校准品和操作程序等构成。对该检测系统所涉及的波长示值误差和重复性、测量线性、检测仪示值误差和重复性、稳定性等进行了系统性研究。测试结果表明,光源示值误差范围为-1.1~6.2nm,光源重复性范围为0.0~0.8nm;线性相关系数R2的范围为0.9930~0.9955;校准品(40.0mg/kg)示值误差范围为0.12%~2.90%,重复性范围为7.15%~8.31%;标准溶液进行测定(6.0mg/L),示值误差范围为-1.92%^-0.87%,重复性范围为1.59%~2.00%;检测仪稳定性为-0.001Abs/h^0.002Abs/h;并将该检测系统应用到肉制品、腌菜等样品中亚硝酸盐含量的现场快速定量测定,从取样到报出检测结果在30分钟内即可完成,与国家标准方法进行了比对,结果令人满意。

A field rapid quantitative detection system for nitrite in food was developed.It consists of instrument,reagent kit,calibrator and operation procedure.The indication error and repeatability for wavelength,linearity,indication error and repeatability for analyzer,stability were discussed.The range of error of indication and repeatability for light source were-1.1nm^6.2nm and 0.0~0.8nm respectively.The Absorbance is directly proportional to the concentration of nitrite and the linear regression equation was obtained with high correlation coefficient（0.9930~0.9955）.The range of error of indication and repeatability for nitrite determination（40.0mg/kg）were-1.92%^-0.87% and 1.59%~2.00% respectively.The stability of instrument was-0.001Abs/h^0.002Abs/h.The detection system has been successfully applied to the determination of nitrite in food,and it costs only 30 minutes from sampling to reporting the test results.The results obtained by the proposed method are in good agreement with the results obtained by the national standard method.