摘要
目的了解2012年北京市某幼儿园手足口病暴发疫情的病原分子特征。方法采集病例咽拭子标本,利用RD细胞和Hep-2细胞进行病毒分离。RT-PCR扩增肠道病毒VP1区基因序列,生物信息学方法鉴定基因型别并进行种系发生分析。结果 13例患者样本中有11件为非肠道病毒71型(EV71)非柯萨奇病毒A16型(CVA16)肠道病毒阳性,其中9例患者咽拭子鉴定为柯萨奇病毒A10型(CVA10),2例样本基因分型失败。9份咽拭子样本分离出7株CVA10毒株。VP1区基因序列分析表明:本起疫情分离株核苷酸和氨基酸的同源性分别为99.8%～100%,99.7%～100%。与2009年山东CVA10分离株VP1区核苷酸和氨基酸的同源性分别为94.8%～96.3%,98.3%～99.3%,与2010年法国CVA10分离株及CVA10原型株(Kowalik株)VP1区核苷酸和氨基酸同源性分别为72.6%～78.1%,91.1%～93.3%。结论本起手足口暴发疫情由CVA10毒株引起。该毒株与山东2009年CVA10分离株亲缘关系较近。
Objective To analyze the etiological characteristics of Coxsackievirus A10 isolated from clinical speci- mens of hand foot and mouth disease. Methods The throat swabs were collected from suspected patients. RD cell and Hep - 2 cell were used to isolate the virus. RT - PCR was used to amplify the VPI gene and the genotype was identified by molecular typing method and bioinformatics analysis. The homogeneity of VP1 from isolated strains was analyzed. Results Eleven specimens were identified as non - enterovirus type 71 （ EV71 ）, non - coxsackievirus A16（ CVA16 ） enteroviurs in 13 specimens from patients, and 9 of the 11 positive specimens were identified as CVAIO. Seven CVA10 strains were isolated from nine CVA10 positive clinical specimens. The homology of nucleo- tide and amino acid among the seven CVA10 strains were 99.8% - 100%, 99.7% - 100%, respectively. Com- pared with the CvA10 strains isolated from Shandong province in 2009, the homology of nucleotide and amino acid were 94.8% - 96.3% ,98.3% - 99.3%, respectively. Compared with the CvA10 strains isolated from France in 2010 and prototype strain of CVA10, the homology of nucleotide and amino acid were 76.2% - 78.1% ,91.1% - 93. 3%, respectively. Conclusion This outbreak of hand foot and mouth disease was induced by CvA10 virus strain which has high homology with the CVA10 strains isolated from Shandong province in 2009.
出处
《中国卫生检验杂志》
北大核心
2013年第10期2279-2282,共4页
Chinese Journal of Health Laboratory Technology
