微量量热技术测定酶催化反应的研究进展

Progress of research on microcalorimetry of enzyme-catalyzed reaction

酶活性和动力学行为是生物化学和催化化学研究中非常重要的内容。本文综述了微量量热技术在酶催化反应中的最新研究和应用进展,重点介绍了等温滴定量热和流动微量量热技术,并讨论了微量量热技术在计算酶催化反应动力学参数方面的优势。

Determination of enzyme kinetics and activity are the most important analytical procedures in biochemistry. The present review deals with the research progress of the microcalorimetry of enzyme- catalyzed reaction. The more recent applications of calorimetric techniques such as isothermal titration cal- orimetry and flow calorimetry to the study of enzyme kinetics, as well as the advantages on using calorime- tric techniques in the determination of kinetic parameters of enzymes, is also discussed here.