河南省汉族人群2型糖尿病患者PLIN基因rs2304796和rs894160位点的多态性

Polymorphism of rs2304796 and rs894160 loci of PLIN gene in patients with type 2 diabetes from Henan Han population

目的：探讨PLIN基因rs2304796及rs894160位点多态性与河南汉族人群2型糖尿病的关系。方法：采用随机整群抽样的方法收集河南省农村地区2型糖尿病患者161人和非糖尿病患者1113人，应用PCR-RFLP和荧光定量PCR技术检测PLIN基因rs2304796和rs894160位点的多态性。用Unphased 3．0．7软件构建单倍型，用logistic回归模型分析该基因变异对2型糖尿病发生的影响。结果：2型糖尿病患者和非患者中PLIN基因rs2304796位点基因型频率和等位基因频率分布差异有统计学意义（χ2＝6．018，P＝0．049；χ2＝5．916，P＝0．015），rs894160位点基因型频率和等位基因频率分布差异无统计学意义（χ2＝0．680和0．295，P＞0．05）。 Logistic回归分析结果显示，在调整了人口统计学特征、肥胖及血脂测量指标后，rs2304796位点CT或TT基因型携带者患2型糖尿病的危险性降低[ OR（95％CI）＝0．670（0．475～0．944）]。结论：PLIN基因rs2304796位点CT或TT基因型可能是2型糖尿病的保护性因素。

Aim：To investigate the polymorphism of rs2304796 and rs894160 loci of PLIN gene in patients with type 2 diabetes from Henan Han population .Methods：A total of 1 274 adults （161 type 2 diabeties and 1 113 controls） were re-cruited.PLIN rs2304796 polymorphism was genotyped by PCR-RFLP and PLIN rs894160 was genotyped by Real-time PCR. The software Unphased 3.0.7 was performed to construct the haplotypes of every individual .Association between PLIN poly-morphism and type 2 diabetes was estimated by logistic regression model adjusted for covariates .Results：Genotype and al-lele frequency of rs2304796 between the 2 populations had statistical difference （χ2 =6.018,P=0.049, χ2 =5.916,P=0.015）.There were no statistical differences in rs894160 genotype and allele frequency between type 2 diabeties and controls （χ2 =0.680,0.295,P〉0.05）.Data from logistic analysis revealed that （CT＋TT）of PLIN gene rs2304796 polymorphism de-creased the risk of type 2 diabetes after adjusting obesity and blood liqid indexes [OR（95%CI）=0.670（0.475-0.944）]. Conclusion：The SNP（CT＋TT）in rs2304796 of PLIN gene might be a protective factor for type 2 diabetes.