miR-106b对HeLa细胞顺铂化疗敏感性的影响

Effects of miR-106b on cisplatin chemosensitivity in HeLa cells

目的:探讨miR-106b对宫颈癌HeLa细胞顺铂化疗敏感性的影响。方法:采用实时定量PCR检测转染miR-106b抑制剂和类似物后HeLa细胞中miR-106b的表达,MTT法及流式细胞仪检测转染后HeLa细胞对顺铂的敏感性和凋亡,Western blot检测细胞中Twist1、PTEN、p-AKT(Ser473)蛋白的表达。结果:miR-106b抑制剂可减少HeLa细胞中miR-106b的表达;而miR-106b类似物可增加HeLa细胞中miR-106b的表达。转染miR-106b抑制剂后,细胞对顺铂的敏感性增强、凋亡及PTEN蛋白表达增加,Twist1、p-AKT表达降低(P<0.05);转染miR-106b类似物后,细胞对顺铂的敏感性减弱,凋亡及PTEN蛋白表达降低,Twist1、p-AKT表达增加(P<0.05)。结论:miR-106b可能通过调节Twist1、PTEN和p-AKT的表达影响HeLa细胞对顺铂耐药。

Aim：To explore the effects of miR-106 b on chemosensitivity of HeLa cells to cisplatin and its possible mechanism.Methods：miR-106b inhibitors and mimics were transiently transfected into HeLa cells , and the expression of miR-106b was detected by real-time PCR.Then, the drug sensitivity of cells to cisplatin cell apoptosis and the expression levels of Twist1, PTEN and p-AKT were detected by MTT assay , flow cytometry and Western blot .Results：The expres-sion of miR-106 b was significantly decreased after the miR-106 b inhibitors transfection , and increased after the mimics transfection in HeLa cells.Moreover, knockdown of miR-106b expression dramatically increased cell chemosensitivity and apoptosis effects induced by cisplatin and the expression of PTEN , but decreased the levels of Twist 1 and p-AKT in HeLa （P〈0.05）.On the contrary, overexpression of miR-106b significantly decreased the cell chemosensitivity and apoptosis effects induced by cisplatin and the expression of PTEN , but enhanced the levels of Twist1 and p-AKT（P〈0.05）.Con-clusion：Cisplatin chemosensitivity of HeLa cells may be modulated by miR-106 b through changing the expressions of Twist1, PTEN and p-AKT.