PRL-3促进子宫内膜腺癌细胞侵袭能力

PRL-3 inhibits migration and invasion of endometrial adenocarcinoma cell lines

目的探讨肝再生磷酸酶（PRL-3）在子宫内膜腺癌细胞中的表达及其与子宫内膜腺癌细胞迁移和侵袭的关系。方法应用逆转录-聚合酶链式反应（RT-PCR）和Western方法检测了PRL-3在2种子宫内膜腺癌细胞系中的表达情况，并在两种细胞中转染PRL-3siRNA24、36、48、60和72h后检测PRL-3的表达情况。同时应用transwell小室观察抑制PRL-3后，两种细胞侵袭能力的变化。采用独立t检验比较实验组与对照组问PRL-3的结果的差异。结果PRL-3在2种子宫内膜腺癌细胞系中均高表达。在两种细胞中，转染PRL-3siRNA36h后，PRL-3表达最低（P〈0．01），并进一步发现，转染后HEC．1-B和AN3CA细胞迁移[细胞数为（18．67士7．49）个、（15．96±8．42）个，与各对照组比较，t值分别为5．23～6．45、3．32～5．33间，P均=0．000]；转染后HEC-1．B和AN3CA细胞侵袭[细胞数为（4．67±0．98）个、（3．89±1．09）个，与各对照组比较，t值分别为4．86～6．98、4．36～5．64间，P均=0．000]。结论PRL-3在子宫内膜腺癌细胞中高表达，且下调PRL-3能够抑制细胞迁移和侵袭能力。

Objective To investigate the expression of PRL-3 in endometrial adenocarcinoma cell lines HEC-1-B and AN3 CA and the relationship between PRL-3 and the migration and invasion of endometrial adenocarcinoma cells. Methods The expression of PRL-3 in the endometrial adenocarcinoma cells after transfection were examined by RT-PCR and Western Blot after a given period time （24, 36, 48, 60 and 72 h after transfection with PRL-3 siRNA） was investigated. Meanwhile, we investigated the effect of PRL-3 on cell migration and invasion ability in endometrial adenocarcinoma cells by transwell. The t-test was used to compare the PRL-3 effect between control group and experimental group. Results The results showed that PRL-3 was highly-expressed in endometrial adenocarcinoma cell lines. The lowest expression of PRL-3 occurred in the cells transfected with PRL-3 siRNA after 36h. Furthermore, downregulating PRL-3 in endometrial adenocarcinoma cells inhibited their their migration ability （the number of cells are 18.67 ± 7.49 and 15.96 ± 8.42 for HEC-1-B and AN3 CA respevtively, t = 5.23 ± 6.45 and t = 3.32 - 5.33, P = 0.000 compare with control group） and invasion ability （the number of cells are 4.67 ± 0.98 and 3.89 ± 1.09 respectively, t = 4.86 - 6.98 and t = 4.36 - 5.64, P = 0.000, compare with control group）. Conclusions PRL-3 was highly-expressed in endometrial adenocarcinoma cells and down-regulating PRL-3 could inhibit cell migration and invasion.