摘要
田间调查了69份烤烟品种形态学性状，并进行各品种间的遗传距离分析；对69份烤烟种质资源进行SSR分析，估算遗传相似系数；结合这2种方法对69份烤烟资源进行遗传多样性研究．结果表明，69份烤烟种质材料间的遗传距离在0.32～7.83之间，平均为2.80，其遗传相似系数在0.30～0.96之间，平均为0.70．聚类分析结果为，供试品种大致分为国外优质种质及其衍生的后代、国内外地方品种、早花类型品种、晚花类型品种等几个类群．结合形态学标记和分子标记对烤烟种质资源进行遗传多样性评价，揭示了烤烟种间的遗传多样性与亲缘关系．
The genetic distance of sixty-nine flue-cured tobacco germplasm resources were analyzed by the field performance characters and SSR markers .The genetic distance was 0.32-7.83 among 69 flue-cured tobacco germplasm varieties , with an average of 2.80.The genetic similarity index was 0.30 -0.96 among 69 flue-cured tobacco varieties , with an average of 0.70 .Cluster analysis based on the ma-trix of genetic distance and genetic similarity index , respectively, was performed.Clustering results showed that the tested tobacco varieties could be divided into foreign varieties , domestic varieties , early-flowering type , late-flowering type etc ..The combination of morphological markers and molecular mark-ers to evaluate the genetic diversity of flue-cured tobacco germplasm resources may reveal the interspecific genetic diversity and genetic relationship of tobacco , providing the theory basis for parental selection in cross breeding of tobacco genetic breeding .
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2013年第4期450-457,共8页
Journal of South China Agricultural University
基金
国家烟草专卖局重点科技项目(110200902043)
广东省烟草专卖局(公司)科技项目(200816)
关键词
烤烟
种质资源
形态学标记
SSR标记
遗传多样性
聚类分析
flue-cured tobacco
germplasm resource
morphological marker
SSR markers
genetic diversity
cluster analysis
