人参皂苷Rb_3对糖尿病模型小鼠的降血糖和抗氧化作用

Effects of Ginsenoside Rb_3 on Antihyperglycemia and Antioxidation in Diabetic Mice

为探讨人参皂苷Rb3对糖尿病治疗的作用及机理,以链脲佐菌素(STZ)诱导的糖尿病小鼠模型为材料,系统分析了人参皂苷Rb3对糖尿病小鼠在降血糖、降血脂、胰岛素调节及抗氧化机能方面的作用.对小鼠禁食血糖浓度的测定显示,人参皂苷Rb3灌胃给药14 d后对糖尿病小鼠具有极显著的降血糖作用(P<0.01),其中剂量为30mg·kg-1的人参皂苷Rb3具有更好的降糖效果.人参皂苷Rb3的降糖效果与糖尿病治疗药物盐酸二甲双胍相近.对小鼠血清中总胆固醇(TC)、三酰甘油(TG)及胰岛素(INS)的含量测定发现,人参皂苷Rb3在降低糖尿病小鼠的血脂浓度和提高胰岛素含量方面均具有显著的调节作用,其中剂量为30 mg·kg-1的人参皂苷Rb3具有更好的疗效,各项治疗指标均优于盐酸二甲双胍.对小鼠抗氧化机能的分析表明,人参皂苷Rb3在提高糖尿病小鼠血液中超氧化物歧化酶(SOD)活力方面具有显著作用,同时可以有效降低糖尿病小鼠血清中丙二醛(MDA)的含量,这对于改善糖尿病小鼠的抗氧化能力和缓解并发症的发生具有重要意义.

In order to investigate the role of ginsenoside Rb 3 in the therapy of diabetes and its mecha-nisms, the streptozocin-induced diabetic mice model was constructed and the effects of ginsenoside Rb 3 on serum glucose , serum lipid , insulin and antioxidative indices were systematically studied .The detec-tion of fasting blood glucose levels indicated that 14 d treatment with ginsenoside Rb 3 via intragastric ad-ministration had a significant （ P〈0 .01 ） hypoglycemic activity on diabetic mice and the concentration of 30 mg· kg -1 had more effective therapy results , which was equivalent to that of diabetic medicine met-formin hydrochloride .The effects of ginsenoside Rb 3 on serum total cholesterol （ TC ） , glycerin trimyr-istate （ TG） and insulin （ INS） were analyzed and the results showed that the Rb 3 had a significant regula-tory function to decrease the concentration of serum lipid , which increased that of insulin in diabetic mice.The dose of 30 mg· kg -1 Rb3 was more effective than that of metformin hydrochloride .The antiox-dative effects of Rb 3 were evaluated and the results illustrated that the activity of superoxide dismutase （SOD） had greatly increased, while the content of malonaldehyde （MDA） had obviously decreased in In order to investigate the role of ginsenoside Rb 3 in the therapy of diabetes and its mecha-nisms, the streptozocin-induced diabetic mice model was constructed and the effects of ginsenoside Rb 3 on serum glucose , serum lipid , insulin and antioxidative indices were systematically studied .The detec-tion of fasting blood glucose levels indicated that 14 d treatment with ginsenoside Rb 3 via intragastric ad-ministration had a significant （ P〈0 .01 ） hypoglycemic activity on diabetic mice and the concentration of 30 mg· kg -1 had more effective therapy results , which was equivalent to that of diabetic medicine met-formin hydrochloride .The effects of ginsenoside Rb 3 on serum total cholesterol （ TC ） , glycerin trimyr-istate （ TG） and insulin （ INS） were analyzed and the results showed that the Rb 3 had a significant regula-tory function to decrease the concentration of serum lipid , which increased that of insulin in diabetic mice.The dose of 30 mg· kg -1 Rb3 was more effective than that of metformin hydrochloride .The antiox-dative effects of Rb 3 were evaluated and the results illustrated that the activity of superoxide dismutase （SOD） had greatly increased, while the content of malonaldehyde （MDA） had obviously decreased in diabetic mice after the treatment of Rb 3 , which was significant in improving the antioxdative ability and relieving diabetic complication .