CC趋化因子受体-2在肝细胞肝癌组织中的表达及临床意义

Expression of CC chemokine receptor 2 in hepatocellular carcinoma and its clinical significance

目的研究CC趋化因子受体-2（CC chemokine receptor2，CCR-2）在肝细胞癌组织中的表达及其与肝癌临床病理特征、预后的关系。方法用免疫组织化学法（SP法）检测76例肝癌组织及其癌旁组织中CCR2的表达情况，分析CCR-2表达与肝癌各项临床病理特征以及患者预后的关系。CCR-2染色结果与各项临床指标的关系采用Х^2检验或Fisher确切概率法检验。采用KaplanMeier法绘制生存曲线。生存情况分析采用lo^rank检验。结果14（18．40A）例癌旁组织CCR-2染色结果显示为弱阳性表达，其余均为阴性。在肝癌组织中，CCR2表达明显高于癌旁组织：15（19．7％）例阴性表达，29（38．2％）例弱阳性表达，12（15．8％）例中等阳性表达，20（26．3％）例强阳性表达。肝癌组织中CCR2阳性表达与肿瘤直径（Х^2=12．41，P〈0．01）、门静脉癌栓（Х^2=7．476，P=0．006）、转移（Х^2=7．227，P=0．007）、AJCC分期（Х^2=20．711，P〈0．01）明显相关。CCR2高表达组患者术后五年生存率（6．3％）明显低于低表达组（38．6％），差异具有统计学意义（Х^2=27．133，P％0．01）。经单因素及多因素分析显示，肝癌组织CCR-2高表达是影响肝癌患者总体生存的独立危险因素（P〈0．05）。结论CCR-2在肝癌组织中表达明显升高，可能参与了肝癌的发生发展过程，检测CCR-2表达水平对判断肝癌的预后具有一定的参考价值。

Objective To investigate the expression of CC chemokine receptor 2 （CCR-2） in hepatocellular carcinoma （HCC） and to evaluate its relationship with the clinicopathological features and prognosis of HCC. Methods Immunohistochemistry was used to detect the expression of CCR 2 in the HCC tissues and their corresponding non-cancerous adjacent liver tissues in 76 patients with HCC. Statistical analysis was used to determine the association of CCR 2 expression with clinicopatho- logical features and prognosis. The expressions of CCR 2 and the relationship between CCR 2 expression and clinicopathological features and prognosis were analyzed using the Chi-square test and Fisher exact probability test. The survival curve was drawn using the Kaplan-Meier method, and the survival was analyzed using the log-rank test. Results Weak positive staining of CCR-2 was detected in the non-cancerous adjacent liver tissues of 14 samples （18.4%） and negative staining was detected in the non-cancerous adjacent liver tissues of the remaining samples. The expression of CCR-2 was different in the HCC tissues： negative CCR-2 was detected in 15（19.7%） samples, weak positive staining of CCR-2 in 29 （38.2%~） samples, moderate positive staining of CCR-2 in 12 （15.8%） samples, and strong positive staining of CCR 2 in 20（26.3%） samples. The high expression of CCR-2 strongly correlated with tumor size （Х^2=12.41,P〈0.01）, venous invasion （Х^2=7. 476,P=0. 006）, metastasis （Х^2=7.227,P=0.007） and AJCC TNM stage （Х^2=20. 711,P〈0. 01）. The cumulative 5 year survival rate was 38.6% in the low CCR-2 expression group, whereas it was 6.3%in the high CCR-2 expression group （Х^2= 27. 133,P〈0.01）. Univariate analysis and multivariate analysis showed that high CCR 2 expression was an independent predictor of overall survival of HCC （P〈0.05）. Conclusions The expression of CCR 2 is highly up-regulated in HCC tissues, indicating that high CCR 2 expression is involved in the process of HCC carcinogenesis and progression, and its expression may serve as a biomarker for predicting prognosis of patients with HCC.