皮肤烧伤愈合过程中磷酸化c-Jun的表达

Expression of phospho-c-Jun during the burned skin wound healing

目的观察烧伤后人皮肤磷酸化c-Jun(p-c-Jun)的变化特点,初步探讨烧伤后创面愈合的分子机制。方法取12例烧伤后住院植皮患者的烧伤周边区皮肤为实验组;另取正常皮肤12例为对照组。应用免疫组织化学方法和常规HE染色检测p-c-Jun在皮肤烧伤周边区和正常皮肤组织中的变化情况。结果对照组p-c-Jun阳性颗粒主要定位于表皮细胞和内皮细胞中,阳性细胞率为(13.9±2.7)%;实验组p-c-Jun阳性颗粒主要定位于表皮细胞和部分炎细胞中,阳性细胞率上升至(47.2±8.6)%,明显高于对照组(P<0.01)。结论烧伤后人皮肤p-c-Jun的表达可能与创面愈合有关。

Objective To observe the change of phosphorylation c-Jun（phospho-c-Jun） during the skin burned wound healing in patients.Methods The staining intensity and distribution of phospho-c-Jun was detected by immunohistochemical and pathological methods in 12 cases with burned skin and 12 cases of normal skin.Results In normal skin,phospho-c-Jun was mainly located in epidermal cells and endothelial cells.The ratio of phospho-c-Jun-positive cells was 13.9±2.7%.In the burned skin cases,phospho-c-Jun immunoreactivity was mainly detected in epidermal cells and some inflammatory cells.The ratio of c-Jun-positive cells was significantly elevated to 47.2±8.6%.Conclusion Increased expression of phospho-c-Jun may be associated with wound healing in burned skin.