急性髓系白血病患者EVI1基因表达的研究

Expression of the EVI1 gene in acute myeloid leukemia

目的建立EVI1基因不同转录本的定量表达检测方法，并研究其在各型急性髓系白血病（AML）中的表达模式，探讨EVI1基因表达与AML发生、预后的关系。方法检测AML患者69例，其中男37例，女32例，年龄10～70岁，中位年龄42岁；按照FAB分类标准，M2 16例，M3 36例，M4 8例。对照为9名健康人。采用荧光定量PCR（Taq Man探针）法检测EVIl基因各转录本的表达，以ABL基因作为内参，计算样本EVI1基因表达差异的倍数（EVI1／ABL），统计分析不同AML患者间EVI1基因的表达差异。结果EVI1基因的不同转录本在所有健康对照样品中均表达，而且不同转录本之间的表达水平差异有统计学意义（P〈0.05）。转录本2和5（同一对引物检测）的表达水平最低，其次为转录本1，再次为转录本6，转录本3的表达水平最高。在健康对照样品中转录本2（5）、1、6及3的中位表达水平分别是阴性、0.005、0.050及0.512。AML患者EVI1基因的表达与融合基因AML-ETO及CBFB-MYH11的表达呈负相关。结论建立了一种稳定、快速、准确的EVI1基因表达的检测方法，而且EVI1基因的表达与AML的预后具有相关性。

Objective Established the method to detect different transcripts of EVI1 gene expression with quantitative PCR and study the expression patterns of EVI1 gene in different leukemia groups to investigate the association between EVI1 gene expression and the incidence and prognosis of leukemia. Methods 60 cases acute myeloid leukemia （AML） and 9 cases normal control were detected in the study, 37 cases were male and 32 cases were female,age 10-70 years, median age 42 years, M3 36 cases, M2 16 cases and M4 8 cases according to FAB classification criteria, control samples of nine cases were normal healthy people. Using the quantitative PCR （Taq Man probe） to detect the expression of different transcripts of EVI1 gene. The t test was used to detect the expression difference among different leukemia groups. Results ABL gene was used as internal reference, relative changes of EVIl gene expression level were detected by EVI1/ABL. In all the control patients, EVIl gene of different transcription of this expression were detected, expression level of EVIl gene different transcription was significant with the difference （P 〈 0.05）, transcription 2 and 5 （the same primers） were the lowest,followed for transcription 1 and 6, expression of transcription 3 was the highest. The expression levels of transcripts 2 and 5, 1, 6, 3 were nagative, 0.005, 0.050 and 0.512 respectively in healthy control samples. In addition, the EVI1 gene expression was negatively correlated with expression of the fusion gene AML-ETO and CBFB-MYH11 in AML. Conclusion The study established a stable, fast and accurate method to detect the expression of EVI1 gene.