负压伤口治疗对小鼠大肠癌模型肿瘤进展的影响

Influence of colorectal cancer progress in mice model with negative pressure wound therapy

目的观察负压伤口治疗对小鼠大肠癌模型肿瘤进展相关指标的影响,探讨负压伤口治疗在肿瘤难愈性伤口中的运用价值及理论依据。方法建立BABL/C小鼠大肠癌伤口动物模型,利用负压吸引进行伤口治疗,实时荧光定量PCR检测治疗后不同时间点肿瘤组织HIF-1α、VEGF、MMP-2 mRNA的表达水平,对比负压伤口治疗小鼠(试验组)与常规治疗小鼠(对照组)肿瘤大小的变化。结果负压治疗后2、4 d试验组肿瘤组织HIF-1αmRNA表达水平比对照组明显升高(P<0.05),至治疗后8 d时HIF-1αmRNA表达水平比对照组明显下调(P<0.05)。同时,治疗后4、8 d试验组肿瘤组织VEGF mRNA表达水平明显高于对照组(P<0.05)。至治疗后14 d时2组HIF-1αmRNA、VEGF mRNA表达比较差异无统计学意义(P>0.05)。试验组肿瘤组织MMP-2 mRNA表达水平持续下降,其不同时间点的表达水平均低于对照组(P<0.05);治疗后14 d对照组出现3例肿瘤转移,而试验组仅有1例转移,试验组肿瘤体积明显大于对照组(P<0.05)。结论负压伤口治疗可以较快改善氧供,且肿瘤不易转移,但同时可促进肿瘤血管生长,使肿瘤体积明显增大。

Objective To explore the influence of negative pressure wound therapy in tumor progression. Methods Establish BABL/C mice colorectal cancer animal models of wound, and use vacuum sealing drainage for wound treatment. The expression of HIF-1α, VEGF, MMP-2 mRNA was measured at different time point by real-time quantitative PCR and the size of tumor was compared between experimental group and control group. Results Expression of HIF-1α mRNA in control group were significantly higher than the trial group at the 2th and 4th day （ P 〈 0. 05 ） , and decreased significantly compared with control group at the 8th day （P 〈 0.05 ）. The expression of VEGF mRNA were significantly higher in trial group at the 4th and 8th day （P 〈0. 05）. At the 14th day, HIF-1α mRNA and VEGF mRNA expression showed no significant difference （ P 〉 0. 05 ）. In the trial group, the expression of MMP-2 mRNA continued to decline and expressed lower than normal group at different time points （ P 〈 0. 05 ）. Modeling after 14 days, 3 cases were found in control group with tumor metastasis, and only 1 case in trial group with bigger tumor volume than control group （P 〈 0. 05 ）. Conclusions Negative pressure wound therapy wound improve the oxygen supply and make the tumor hard to transfer. However, it could accelerate the growth of tumor blood vessels and significantly increase the volume of tumor.