利斯的明立体拆分菌株季也蒙毕赤酵母(Meyerozyma guilliermondii)培养条件对手性纯度和转化率的影响

The Influence of Rivastigmine Stereo-resolving Strain Culturing Conditions on the ee Value and Conversion Ratio

前期研究发现培养条件对利斯的明立体拆分的手性纯度和转化率影响很大,笔者就利斯的明立体拆分菌株季也蒙毕赤酵母(Meyerozyma guilliermondii)HM988686.1的培养基组成(碳源、氮源)和其他工艺条件(培养温度、初始pH、装液量)进行了研究.通过单因素试验和正交试验,确定了该菌株的最适培养条件为蔗糖2%,蛋白胨1%,玉米浆0.5%,pH 8.0,Tris-HCl缓冲体系,培养温度28℃,装液量为100 mL/500 mL三角瓶.在最优的条件下,转化率为42%,产物ee值达95.1%.

An isolated stain Meyerozyma guilliermondii HM988686.1 was found to be able to stereo-resolve riva- stigmine intermediates. The compositions of its culture medium （both carton and nitrogen sources） and other technological conditions （temperature,pH and capacity in shaking flask） were investigated. Through single fac- tor and orthogonal experiments, the optimum culturing conditions of this strain were determined as follows： su- crose 2% , peptone 1% , corn steep liquor 0.5% , pH 8.0, temperature 28 ℃, and 100mL medium in a 500 mL shaking-flask. Under the optimum conditions, the conversion ratio was 42% and the ee value reaches 95.1%.