期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

氯化甲基汞对仔鼠小脑PKCα mRNA表达的影响 被引量：1

Effects of Methylmercury chloride exposure on PKCα mRNA expression in rat brain during development
下载PDF
导出
摘要 目的:观察氯化甲基汞对仔鼠小脑中PKCαmRNA表达的影响。方法:建立氯化甲基汞致仔鼠小脑发育损伤大鼠实验模型。采用核酸原位杂交方法检测α型蛋白激酶C(PCKα)mRNA表达。结果:和对照组相比,各实验组仔鼠在出生后3天至出生后30天,小脑蒲肯野细胞PKCαmRNA表达均明显增高。小脑蒲肯野细胞PKCαmRNA表达随出生后时间的延长,其表达量逐渐增多。并且氯化甲基汞浓度与PKCαmRNA表达量呈正比,随氯化甲基汞浓度增高,小脑蒲肯野细胞PKCαmRNA表达明显增多。结论:氯化甲基汞以剂量依赖方式和时间依赖方式促进仔鼠小脑蒲肯野细胞PKCαmRNA表达。氯化甲基汞可通过上调神经元中丝氨酸/苏氨酸蛋白激酶促进小脑神经元凋亡进而损伤中枢神经系统。 Objective：To investigate the effect of Methylmercury chloride exposure on PKCα mRNA expression in rat brain during development.Methods：Establishment of developmental brain injured model of rats by chronic MMC exposure.In Situ Hybridization analysis were performed to detect the expression of PKC isozyme.Results：PKCα was expressed at low levels at birth but increases significantly during postnatal development.With the increasing brain Hg2＋level,expression of PKCα in rat＇s brain from experimental groups was significantly higher than that of control group.Conclusion：Methylmercury chloride exposure can affect PKCα mRNA expression in rat brain during development,which may results in the increasing of cerebellum neuron apoptosis mediated by serinethreonine kinase pathway.
作者 郭杰 张宏宇 张雯艳 李志超 王铁君 邢程
机构地区 吉林大学第二医院放疗科 中国人民解放军 吉林省妇幼保健院 吉林大学公共卫生学院 吉林省卫生厅
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2013年第9期923-925,共3页 Chinese Journal of Immunology
基金 吉林省卫生厅科研基金资助项目(3D5097923427) 吉林大学基本科研业务费专项基金资助项目(421030140427) 吉林大学青年教师基金资助项目(419070210048)
关键词 氯化甲基汞 脑发育 PKCαmRNA Methylmercury chloride Brain development PKCα mRNA
分类号 R392 [医药卫生—免疫学]
  • 相关文献

参考文献16

  • 1郭杰,王铁君,张雯艳,邢程,李志超,管清香.氯化甲基汞对发育阶段大鼠海马组织蛋白激酶C活性的影响[J].吉林大学学报（医学版）,2010,36(5):922-925. 被引量：5
  • 2Xu B, Xu Z F, Deng Yet al. MK-801 protects against Intracellular Ca2+. overloading and improves N-methyl-d-aspartate receptor expres- sion in cerebral cortex of methylmercury-poisoned rats [ J ]. J Mol Neuro, 2013 ; 49( 1 ) : 162-171.
  • 3Farina M, Aschner M, Rocha J B. Oxidative stress in MeHg-induced neurotoxicity [ J ]. Toxicol Appl Pharmacol, 2011; 256 ( 3 ) : 405-417.
  • 4Yasuyosbi Y, Freesia L H, Hiroki N et al. Tissue distribution and developmental expression of protein kinase C isozymes [ J ]. J Biol Chem, 1994; 263(20) : 9868-9873.
  • 5Myers G J, Davidson P W, Cox C et al. Prenatal methylmercury ex- posure from ocean fish consumption in the Seychelles child develop- ment study[ J]. Laneet, 2003 ; (361) : 1686-1692.
  • 6Anna F, Castoldi,Teresa Coceini, Sandra Ceccatelli et al. Neurotox- icity and molecular effects of methylmercury[ J]. Brain Res Bulleti, 2001 ;55(2) :197-203.
  • 7董丽波,李志超,毕晓颖,凌玲,郭丽.氯化甲基汞对发育阶段大鼠脑核因子kappaBDNA结合活性的影响[J].卫生研究,2001,30(1):7-9. 被引量：5
  • 8Kunimoto M. Methylmercury induces apoptosis of rat cerebellar neu- rons in primary culture[ J]. Biochem Biophys Res Commun, 1994; 204( 1 ) :310-317.
  • 9Trom P O, Hinm an L E, Unger Get al. Awound-induced [ Ca2+ ] I increase and its transcriptional activation of immecliate early genes is important in the regulation of motility [ J ]. Expcell Res, 1999 ;246 : 319-326.
  • 10Soh J W, Lee E H, Prywes R et al. Noval roles of specific isoforms of protein kinase C in activation of the c-fos serum response element [ J]. Mol cell Biol, 1999 ; 19 : 1313-1324.

二级参考文献26

  • 1Ying Wen Wei Cui Pei Liu.TypeⅠinositol 1, 4, 5-triphosphate receptors increase in kidney of mice with fulminant hepatic failure[J].World Journal of Gastroenterology,2007,13(16):2344-2348. 被引量：7
  • 2Castoldi AF, Onishchenko N, Johansson C, et al. Neurodevelopmental toxicity of methylmercury: Laboratory animal data and their contribution to human risk assessment [J]. Regul Toxicol Pharmacol, 2008, 51 (2) :215- 229.
  • 3Purkayastha S, Fernando SS, Diallo S, et al. Regulation of protein kinase C isozymes during early postnatal hippocampal development [J].Brain Res, 2009, 1288: 29-41.
  • 4Al-KhairiI, Baharnoori M, Kamath A, et al. Altered expression and alpha-1 adrenergic receptor mediated activity of protein kinase C in the prefrontal cortex of rats with neonatal ventral hippocampus lesions [J]. Synapse, 2009, 63 (12): 1051-1059.
  • 5Russell C, Acevedo2Duncan M. Effects of the PKC inhibitor PD 406976 on cell cycle rogression, proliferation, PKC isozymes and apoptosis in glioma and SVGzt ransformed glial ceils [J]. Cell Prolif, 2005, 8 (2): 87- 106.
  • 6Garrido-Gareia A, Andres-Pans B, Duran-Trio L, et al. Activity dependent translocation of neurogranin to neuronal nuclei[J]. BiochemJ, 2009, 424 (3): 419-429.
  • 7Bradford MM. A rapid and sensitive met hod for thequantitation of microgram quantities of protein utilizing theprinciple of protein dye binding[J]. Anal Biochem, 1976, 72:248 -254.
  • 8Takai Y, Kishimoto A, Iwasa Y, et al. Calcium dependent activation of multifunetional protein kinase by membrane phospholipids [J]. J Biol Chem, 1979, 254 (10): 3692-3695.
  • 9Jane EP, Pollack IF. Enzastaurin induces H2AX phosphorylation to regulate apoptosis via MAPK signalling in malignant gliorna cells [J].EurJ Cancer, 2010, 46 (2): 412- 419.
  • 10Karpova LV, Bulygina ER, Boldyrev AA. Different neuronal Na (+) /K (+) -ATPase isoforms are involved in diverse signaling pathways [J]. Cell Bioehem Funet, 2010, 28 (2): 135-141.

共引文献18

同被引文献14

  • 1Boischio A. Developmental neurotoxicity: methylmercury and prenatal exposure protection in the context of the Minamata Convention [ J ]. Rev Panam Salud Publica, 2015, 38 ( 3 ) : 243 -247.
  • 2Farina M, Aschner M, Rocha JB. Oxidative stress in MeHg-induced neurotoxicity [ J ]. Toxicol Appl Pharmacol, 2011,256 ( 3 ) : 405-417.
  • 3Sadiq S, Ghazala Z, Chowdhury A, et al. Metal toxicity at the synapse : presynaptic, postsynaptic, and long-term effects [ J ]. J Toxicol, 2012,2012 : 132671 - 132682.
  • 4Berardi DE, Bessone MI, Motter A,et al. Involvement of protein kinase C α and δ activities on the induction of theretinoic acid system in mammary cancer ceils[ J]. Mol Carcinog,2015,54(10) : 1110-1121.
  • 5Xu B,Xu ZF, Deng Y, et al. MK-801 protects against Intracellular Ca^2+ overloading and improves N-methl-d-aspartatereceptor expression in cerebral cortex of methylmercury-poisoned rats [ J ]. J Mol Neuro,2013,49( 1 ) :162-171.
  • 6Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding[ J ]. Anal Biochem, 1976, 72:248-254.
  • 7Yasuyoshi Y, Freesia LH, Hiroki N, et al. Tissue distribution and developmental expression of protein kinase C isozymes[ J]. J Biol Chem, 1994,263 (20) :9868-9873.
  • 8Kunimoto M. Methylmercury induces apoptosis of rat cerebellar neurons in primary culture [ J ]. Biochem Biophys Res Commun, 1994,204( 1 ) :310-317.
  • 9Soh JW, Lee EH, Prywes R, et al. Noval roles of specific isoforms of protein kinase C in activation of the c-fos serum response element[ J ]. Mol cell Biol, 1999,19 : 1313-1324.
  • 10Leverrier S, Vallentin A, Joubert D. Positive feedback of protein kinase C proteolytic activation during apoptosis [ J ]. Biochem J, 2002,368 ( Pt 3 ) :905-913.

引证文献1

二级引证文献1

中国免疫学杂志
2013年 第9期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部