阿魏酸甲酯一氧化氮供体在小鼠体内的药动学参数测定

Pharmacokinetics Parameters of Ferulic Acid Methylester Nitric Oxide Donor in Mice

目的:建立阿魏酸甲酯一氧化氮供体(FMND)的反相高效液相色谱分析方法,并对其在小鼠体内的药动学参数进行测定。方法:以乙酸乙酯提取后,漩涡,高速离心,氮气吹干,流动相复溶后进样。色谱条件:流动相为甲醇:水(70∶30),流速:1.0 mL/min。柱温:30℃。结果:小鼠灌胃给予50 mg/kg的FMND后吸收较快,迟滞时间为6.6 min,约1.5 h达到血药浓度高峰。体内分布符合有时滞的二室模型。分布半衰期为1.04 h,消除半衰期为9.44 h,吸收半衰期为0.94 h。结论:阿魏酸甲酯一氧化氮供体在小鼠体内吸收较快,分布平衡相对较快,消除半衰期较长。

Objective： To develop the assay method by RP-HPLC for ferulic acid methylester nitric oxide donor （FMND） and determine the pharmacokinetics parameters in mice after oral administration. Methods： The sample was extracted by acetoacetate, followed by vortex and high speed centrifugation, dried by nitrogen flow and dissolved by mobile phase before HPLC analysis. The HPLC condition was applied with mobile phase at methanol： water （70：30）, flow rate at 1.0 mL/min, column temperature at 30 ℃. Results： FMND was absorbed quickly with a lag time of 6.6 min after oral administration in mice at the dose of 50 mg/kg, and arrived to the peak concentration at about 1.5 h. The distribution in vivo matched two-compartment model with a lag time. The distribute half life time （t1/2α） was 1.04 h, the eliminate half life time （t1/2 β） was 9.44 h, the absorb half life time （t1/2 Ka） was 0.94 h. Conclusion： FMND was absorbed quickly in mice after oral administration, and the distribute equilibrium arrived moderately with a long eliminate half life time.