河南省农田土壤芽孢杆菌的遗传多样性分析

Genetic Diversity of Bacillus in Agricultural Soils in Henan Province

从河南省不同地区的农田土壤中分离纯化需氧芽孢杆菌,通过16SrRNA基因的系统发育分析,在分子水平上深入研究了河南省芽孢杆菌的物种类型及其遗传多样性。共获得331株产芽孢菌株,分属于19个物种,其中1株为芽孢杆菌属内的潜在新物种。巨大芽孢杆菌、简单芽孢杆菌、枯草芽孢杆菌和蕈状芽孢杆菌为全省范围内的优势菌种,分别占总菌株数量的37%、22%、15%和12%。河南省内不同地区的优势类群略有差异,并且还存在各自独特的芽孢杆菌物种。从分子水平证明河南省蕴藏着丰富的芽孢杆菌资源。

Bacillus is a kind of valuable biological resource. In this study, the species and genetic diversity of bacillus was investigated in agricultural soils in Henan province based on the phyloge- netic analysis of 16S rRNA gene. Total 331 strains were isolated and belonged to 19 species of Ba- cillaeeae,in which one was a candidate novel Bacillus species. The species Bacillus megaterium, Bacillus simpleac, Bacillus subtilis and Bacillus mycoides were predominant in Henan province and represented 37%,22%,15% and 12% of total strains,respectively. For different regions in the province,they had some difference in the predominant species,and harbored some particular species for themselves. In summary,the present study proves that Henan province has rich baci- llus resources.