iNOS和OPN在EAE中动态表达及依达拉奉保护作用研究

The dynamic expression of iNOS and OPN in experimental autoimmune encephalomyelitis and the protection of edaravone

目的研究诱导型一氧化氮合酶(iNOS)和骨桥蛋白(OPN)在实验性自身免疫性脑脊髓炎(EAE)中的动态表达以及依达拉奉治疗和保护作用机制的探讨。方法将EAE大鼠随机分为实验组和依达拉奉治疗组,根据发病时间又分为发病前组、高峰期组和缓解期组。光镜下观察脊髓HE染色炎性细胞浸润情况,观察免疫组化染色iNOS和OPN阳性细胞数目。结果依达拉奉治疗组与实验组比较,发病时间延迟、发病率降低及神经功能评分减低(P<0.05)。依达拉奉治疗组脊髓炎症细胞浸润较实验组减少。免疫组化染色iNOS和OPN阳性细胞在EAE发病前期上升,高峰期达到峰值,缓解期随疾病好转而下降,依达拉奉组均较同时期实验组阳性细胞表达数目减少(P<0.05)。结论依达拉奉可以减轻EAE大鼠临床发病程度和病理炎症损害,并降低不同发病时期iNOS和OPN表达程度。推测依达拉奉通过抗氧化和抗炎的双重机制发挥神经保护作用。

Objective Detecting the expression of inducible nitric oxide synthase （iNOS） and osteopontin （OPN） in different course of experimental autoimmune encephalomyelitis （EAE）,and discussing protective role of edaravone and its probable mechanism.Methods EAE rats were divided into two groups：experimental group and edaravone group.According to course,they were divided into pro-disease group,the third day group（the peak time） and the seventh day group（remission stage）.Results The onset time of rats were postponed in edaravone group compared with experimental group,and decrease of morbidity and clinical neurological score were more obvious in edaravone group than that in EAE group （P 〈0.05）.Infiltration of inflammatory cells and expression of iNOS and OPN were most obvious in third day group than in pro-disease group and the seventh day group（P 〈0.05）.After treatment of edaravone,expression of positive cells of iNOS and OPN were decreased （P 〈 0.05）.Conclusion edaravone can alleviate clinical aad pathological severity of EAE rats and reduce the expression of iNOS and OPN ; it may have double roles of anti-oxidative and anti-inflammatory.