不同饲养环境对局灶性脑梗死大鼠梗死灶周围VEGF表达的影响

The effects of different environmental intervention on VEGF expression in rats after unilateral local cerebral infarction

目的观察不同环境设置对局灶性脑梗死大鼠行为学恢复及梗死灶周围血管内皮生长因子(VEGF)表达的影响,为临床应用tr 6234丰富环境、探索学习等不同环境进行脑卒中康复治疗提供基础理论支持和实验依据。方法将实验动物随机分为假手术对照组(15只)和大脑中动脉阻塞(MCAO)组(90只)。手术组用电凝法造成右侧MCAO模型。MCAO组大鼠于MCAO手术后,再随机分为社会交往组(30只)、探索学习组(30只)、和丰富环境组(30只)。社会交往组5只一组,群居于标准笼;探索学习组10只一组,居于迷宫笼;丰富环境组30只居于丰富环境笼。MCAO各组分别于术后1d、3d、7d、14d、28d随机选取5只,假手术组于术后1d、3d、7d随机选取5只大鼠处死,剖颅取脑,石蜡包埋,切片,免疫组化染色,测定VEGF在脑梗死灶周围皮质的表达情况。结果 VEGF的表达,社交组MCAO术后1d可见梗死灶周围VEGF略有表达,3d表达略有增加(P<0.05)。MCAO各组可见术后第2w时表达增高明显,且均高于假手术组,(P<0.05)。随时间推移,至MCAO后28d时,丰富环境组、探索学习组及社会交往组VEGF表达略有下降。在14d时,丰富环境显著高于探索学习及社会交往组(P<0.05)。结论丰富环境、探索学习可上调局灶性脑梗死后大鼠梗死灶周围皮层VEGF的表达,这种上调可能是丰富环境、探索学习促进大鼠行为学功能恢复原因。

Objective The experiment intended to observe the effects of the different environmental on the behavioral recovery and vascular endothelial growth factor （VEGF） around the infarct in rats with unilateral local cerebral infarction in order to offer the basic theoretical support and experimental instruction for stroke rehabilitation.Methods One hundred and five male SD rats,3 ～ 4 months,weight 240 ～ 260g,were divided into sham （n =15） and MCAO（middle cerebral artery occlusion） groups（n =90）.The rats in MCAO group were subjected to electric coagulation of MCA and then were assigned randomly to ①social commun-ication group（n =30,5 as a group housed in a standard cage）,②learn training group（n =30,10 as a group housed in a maze cage）,③rich environment group（n=30,housed in a rich environment cage）.5 rats from each operated group at 1d,3d,7d,14d,and 28d after MCAO and 5 rats from sham group at 1d,3d,7dwere randomly assigned to be killed.The expression of VEGF in peri-ischemic cortex were examined from paraffin slice and immunohistochemistry staining.Results Immunohistochemistry staining：the expression of VEGF in the peri-ischemia cortex：the positive cells of VEGF was higher than sham group on MCAO groups on day 3 （P 〈0.05）.The rich environment group were significant higher than social communicate and learn traning groups （P 〈0.05） on day 14.The positive cells of VEGF on MCAO group gradually decrease on day 28 but were still higher than sham group.（P 〈0.05）.Conclusion Learn training and enriched environment after MCAO could up-regulate the expression of VEGF in the peri-ischemic regions,and the up-regulation might contribute to the improvement of the recovery of extremity functions resulted from learn training and the enriched environment.