Goto-Kakizaki大鼠十二指肠空肠旷置术模型的建立

Establishment of Duodenal-Jejunal Bypass Animal Model in Goto-Kakizaki Rat

目的探讨以Goto-Kakizaki(GK)大鼠建立保留全胃的十二指肠空肠旷置术(DJB)模型的可行性及操作要点。方法将16只GK大鼠随机分为实验组(n=8)和对照组(n=8)。在规范的术前、术中及术后操作下,对实验组和对照组大鼠分别行DJB术和假手术,比较2组大鼠术前,术后1、2、3及4周空腹血糖和体质量的变化情况,评价手术造模是否成功,并观察2组大鼠的存活情况。结果术后4周2组大鼠均全部存活。与术前比较,实验组术后1周空腹血糖即下降(P<0.05),术后2、3及4周血糖趋于稳定;对照组术后各时相空腹血糖水平无明显变化(P>0.05)。术后各时相实验组的空腹血糖水平均低于对照组(P<0.05),提示成功建立了模型。实验组术后4周体质量的增加值低于对照组(P<0.05)。结论 DJB是一种可行的、安全有效的降糖术式。规范实验操作程序可以降低术中风险,建立稳定的GK大鼠DJB模型。

Objective To explore the feasibility and operation points of establishing duodenal-jejunal bypass （DJB） surgery animal model in Goto-Kakizaki （GK） rats. Methods Sixteen GK rats were randomly divided into experimental group （n=8） and control group （n=8）. In a standardized preoperative, intraoperative, and postoperative operation, the rats of experimental group and control group received DJB and sham surgery respectively. The fasting plasma glucose and body mass were observed before operation, and 1, 2, 3, and 4 weeks after operation in order to evaluate whether the models were established successfully. Survival situation of rats were observed too. Results All experimental rats survived at 4 weeks after the operation. Compared with the levels before operation, the fasting plasma glucose levels of experimental group decreased significantly （/9〈 0. 05） at 1 week after operation, and remained stable at 2, 3, and 4 weeks after operation. The fasting plasma glucose levels of control group did not change statistically at all time points after operation （P 〉 0.05）. Compared with control group at the same time point, the fasting plasma glucose level of experimental group was lower （P〈0.05）, indicating that DJB models were established successfully. After 4 weeks, the value of body mass added in experimental group was significantly lower than those of control group （P 〈 0.05）. Conclusions DJB is a feasible, safe, and effective bypoglycemic surgery. The application of this set of experimental operating procedures can reduce the risk of intraoperative and postoperative mortality, and can develop a stable DJB model in Goto-Kakizaki rats.