原发性肝细胞癌CDC25A的表达与多普勒超声影像特征的关联性分析

Correlation between expression of CDC25A and Doppler ultrasound features in primary hepatocellular carcinoma

目的:检测细胞分裂周期蛋白25A(cell division cycle 25A,CDC25A)基因在原发性肝细胞癌组织中的表达,探讨其与肝癌多勒超声影像特征的关联.方法:运用RT-PCR和Western blot的方法检测86例肝癌组织及对应癌旁组织中CDC25A基因的表达;用B超观察肝癌患者肝脏肿瘤影像学特征.结果:在86例肝癌病例中,75.58%(65/86)的肝癌组织CDC25A mRNA和蛋白表达明显高于对应癌旁组织(P<0.05);肝癌组织中CDC25A的表达与多普勒超声影像特征如肿瘤的大小、血流分级、血流阻力指数及门静脉侵袭情况明显相关(P<0.05),与肝脏肿瘤结节的个数无关(P>0.05).结论:CDC25A基因可能在肝癌的发生及转移过程中发挥重要作用,肝癌组织中CDC25A基因的表达与超声影像相结合可为肝癌临床治疗及预后判断提供理论依据.

AIM： To investigate the expression of cell division cycle 25A （CDC25A） in primary hepatocellular carcinoma （PHC） and to explore its relationship with Doppler ultrasound imaging features. METHODS： The expression of CDC25A in 86 PHC specimens and matched tumor-adjacent tissue specimens was detected by RT-PCR and Western blot. The imaging features of PHC was analyzed by Doppler ultrasound. RESULTS： CDC25A was found to be overexpressed in 75.58% （65/86） of PHC cases （P 〈 0.05）. The expression of CDC25A protein was signi？cantly correlated with Doppler ultrasound imaging features including tumor size, blood flow grade, resistance index （RI） and portal vein invasion （all P 〈 0.05）, but not with the number of liver tumor nodes （P 〉 0.05）. CONCLUSION： CDC25A may play an important role in the development and metastasis of PHC. Detection of CDC25A expression combined with ultrasound imaging may be used clinically for evaluation of treatment response and prognosis in PHC patients.