白术内酯对小鼠结肠癌细胞增殖能力的影响

Atractylenolide Ⅱ significantly reduces proliferation of mouse colon cancer cells

目的:观察白术内酯Ⅰ、Ⅱ、Ⅲ对小鼠结肠癌细胞增殖能力的影响.方法:CT26细胞种植于96孔板,24 h后分别加入白术内酯Ⅰ、Ⅱ、Ⅲ200、100、50、25、12.5、6.25μg/mL,放置培养箱48 h后,MTT法检测白术内酯Ⅰ、Ⅱ、Ⅲ不同剂量对CT26细胞增殖能力的影响;筛选对CT26抑制效果好的白术有效成分,检测其不同剂量给药后24、48、72 h后CT26细胞增殖能力的影响.结果:白术内酯Ⅱ抑制CT26细胞增殖能力最为明显,其最合适给药剂量为50μg/mL,最佳给药时间为48 h.结论:白术内酯Ⅱ可能是抑制肿瘤细胞增殖的主要成分之一.

AIM： To observe the effect of atractylenolides Ⅰ, Ⅱ, and Ⅲ on the proliferation of mouse colon cancer cells. METHODS： CT26 cells were seeded in 96-well plates and treated with different concentrations of atractylenolide Ⅰ, Ⅱ, or Ⅲ （200, 100, 50, 25, 12.5, or 6.25 μg/mL） for 24 h. After culture for an additional 48 h, the proliferation of CT26 cells was detected by MTT assay to screen atractylodes＇ active ingredient that had the best inhibitory effect. CT26 cells were then treated with the screened atractylodes＇ active ingredient at different doses for different durations （24, 48, 72 h）. RESULTS： Atractylenolide II had the best inhibitory effect on the proliferation of CT26 cells. The optimal dose was 50 μg/mL, and the best delivery time was 48 h. CONCLUSION： Atractylenolide Ⅱ can significantly reduce the proliferation of mouse colon cancer cells.