不同靶点注射骨髓基质干细胞对大鼠脊髓损伤修复的影响

Effects on the repair of spinal cord injury in rats with different targets of injection of bone marrow stromal stem cells

目的研究不同靶点注射骨髓基质干细胞(BMSCs)对脊髓损伤(SCI)修复的影响,观察注射的BMSCs在SCI局部和两侧健康组织中的存活情况。方法体外分离培养BMSCs并鉴定。采用改良Allen打击法制作SD大鼠SCI(T10节段)模型,伤后9 d注射细胞,将100只SD大鼠随机分为5组(每组20只):4点法治疗组(脊髓损伤区头、尾侧);6点法治疗组(加上脊髓损伤中心区):8点法治疗组(加损伤区及损伤区头尾的上或下节段);10点法治疗组(加损伤区及损伤区头尾的上和下节段);E组为空白对照组,未注射细胞。通过改良的BBB评分,评估不同靶点注射BMSCs对SCI运动功能恢复的促进作用。通过组织切片和HE染色观察注射细胞在SCI局部和两侧健康组织中的存活情况。结果成功培养BMSCs并鉴定。改良的BBB评分显示,局部注射BMSCs能明显的促进SCI动物的运动功能的恢复(P<0.01)。6、8、10点法注射明显优于4点法(P<0.01)。6、8、10点法之间差异无统计学意义(P>0.05)。组织切片观察显示,6点法注射的BMSCs在SCI局部和两侧健康组织中的存活比例明显优于4点法(P<0.01),6、8、10点法之间差异无统计学意义(P>0.05)。结论 6点法注射能有效的促进SCI动物的运动功能的恢复,同时能减少过多次数的注射对健康脊髓的损伤。注射的BMSCs在SCI局部和两侧健康组织中的存活较好。

Objective To study the different targets bone marrow stromal stem cells （BMSCs） injected to the influ- ence of spinal cord injury（SCI） repair, and to observe the cell injection in spinal cord injury local and on both sides of the health organization of survival situation. Methods In vitro separation culture BMSCs and appraisal. Modified Allen＇s strike legal system for SD rat spinal cord injury （T10 segments） model, the injury after 9d injection cells, will only 100 SD rats were randomly divided into 5 groups （each group 20） ： group A for four points method （spinal cord injury area head, tail side） ; Group B for six points method （plus spinal cord injury center） ; group C for eight points method （plus damage area and damage area head to tail on or next section） ; Group D for ten points method （plus damage area and damage area head and tail of the up and down section） ; group E for blank control group, not injec- ted cells. Through the improvement of BBB rating, evaluate different targets BMSCs injected for SCI the promoting function of movement function recovery. Through the tissue slice and HE staining to observe injection cells in the SCI local and on both sides of the health organization of survival situation. Results Culture BMSCs and appraisal Im- proved BBB score shows local injections of cells can significantly enhance the recovery of movement function of SCI animal （P 〈0. 01 ）. 6, 8, 10 points injection significantly superior to 4 points （P 〈0. 01）. But differences between 6, 8, 10 points methods had no statistical significance （P 〉 0.05）. Tissue section observations showed that percent- age of their survival by 6 points method injection of BMSCs in SCI and health organizations significantly superior to 4 points method （P 〈 0. 01 ）. But differences between 6, 8, 10 points methods had no statistical significance （P 〉 0.05）. Conclusions 6 points method can effectively enhance the recovery of movement function of SCI animal, at the same time can reduce the excessive number of injection of the SCI to health. Local injection of BMSCs in SCI and health organizations on both sides of the live better.