星状神经节阻滞对自发性高血压大鼠心肌细胞凋亡与Bcl-2,Bax蛋白表达的影响

Effect of stellate ganglion block on cardiomyocyte apoptosis and expression of Bcl-2/Bax protein in spontaneously hypertensive rats

目的:研究自发性高血压大鼠(SHR)心肌细胞凋亡与Bcl-1/Bax蛋白表达的关系,探讨星状神经节阻滞对SHR心肌细胞凋亡的保护作用。方法:将32只10周龄雄性SHR随机分为4组(n=8):左侧星状神经节阻滞组(LS组)、右侧星状节阻滞组(RS组)、药物卡托普利组(D组)、手术对照组(C组)。用ALC-NIBP无创血压测量系统测定大鼠血压,第10周实验结束后用3%戊巴比妥钠腹腔注射(45 mg/kg)麻醉SHR,取出心脏用TUNEL法测定左室心肌细胞凋亡指数,免疫组织化学检测心肌Bcl-2和Bax表达的含量。结果:与C组和LS组比较,RS组SHR凋亡指数明显降低(P<0.05);心肌Bcl-2表达明显增强(P<0.05),Bax表达明显减弱(P<0.05),Bcl-2/Bax比值增高(P<0.05)。结论:Bcl-2抗凋亡及Bax促凋亡蛋白表达参与SHR心肌细胞凋亡;右侧星状神经节阻滞能够影响凋亡相关基因蛋白的表达,降低心肌细胞凋亡指数,减轻左心室肥厚。

Objective：To investigate the relationship between apoptosis of myocardial cells in spontaneously hypertensive rats （SHRs） and the expressions of Bcl-2 and Bax protein, and the protective effect of stellate ganglion block on apoptosis of myocardial cells. Methods：A total of 32 ten-week-old male SHRs were assigned randomly into 4 groups：a left stellate ganglion block group （group LS）, a right stellate ganglion block group （group RS）, a captopril group （group D） and a control group （group C）. The arterial systolic blood pressure was measured by ALC-NIBP system. After 10 weeks, all rats were anaesthetized by 3%pentobarbital sodium, cardiomyocyte apoptosis index of left ventricle was assessed by TUNEL, and the localization of myocardium Bcl-2, Bax was investigated by immunohistochemistry. Results：Compared with group LS and C, the apoptotic index decreased （P〈0.05）. SHR myocardial expression of Bcl-2 signiifcantly increased （P〈0.05）, Bax expression signiifcantly decreased （P〈0.05） and Bcl-2/Bax was signiifcantly higher （P〈0. 05） in group RS. Conclusion：Bcl-2 and Bax play an important role in the apoptosis of myocardial cells in SHRs. Right stellate ganglion block can reduce the apoptosis of myocardial cells and reverse the reconstruction of the left ventricle in SHRs via regulation of apoptosis-related gene proteins.