菟丝子水提物对肾间质纤维化大鼠肾组织保护作用的研究

Effect of Cuscutae Semen extract on renal interstitial fibrosis in rats

目的探讨菟丝子水提物对大鼠单侧输尿管结扎诱导的肾间质纤维化的疗效。方法 SPF级SD大鼠随机分为3组:假手术组、模型组、菟丝子组。通过复制肾间质纤维化大鼠模型,检测模型大鼠血清肌酐和尿素氮水平,观察肾组织病理变化,并采用免疫组化技术检测大鼠肾组织α-平滑肌动蛋白(α-SMA)、结缔组织生长因子(CTGF)、I型胶原(Col I)等表达情况。结果相比于模型组,菟丝子给药组大鼠血清肌酐和尿素氮水平显著降低,减少肾间质胶原沉积和肾组织α-SMA、CTGF、Col I表达。结论菟丝子水提物对肾间质纤维化大鼠肾组织有缓解保护作用。

AIM To investigate the effect and mechanism of Cuscutae Semen extract on rat renal interstitial fi- brosis caused by unilateral ureteral obstruction. MEETHODS Forty-eight SPF SD rats were randomly divided in- to sham-operated group, untreated unilateral ureteral obstruction group and Cuscutae Semen-treated unilateral ure- teral obstruction group. Renal function was evaluated by determining serum creatinine and blood urea nitrogen. Haematoxylin ＆ eosin （HE） and Masson staining were used to observe the dynamic histological changes of the kid- neys. The expressions of α-smooth muscle actin （α-SMA）, connective tissue growth factor （CTGF） and collagen type I （Col I） were analyzed by immunohistochemistry staining. RESULTS Compared with untreated unilateral ureteral obstruction group, Cuscutae Semen extract significantly reduced the level of serum creatinine and blood urea nitrogen, decreased interstitial collagen deposition and attenuated the expressions of α-SMA, CTGF and Col I in the obstructed kidneys in Cuscutae Semen-treated unilateral ureteral obstruction group. CONCLUSION The experi- mental finding reveals that Cuscutae Semen extract can retard the progression of renal interstitial fibrosis.