摘要
为解决竹红菌甲素(hypocrellinA,HA)易溶于有机溶剂却极难溶于水的问题,采用物理包埋法,以Triton-X-100为载体,制备了对水稀释后稳定性良好的500mg/L的竹红菌甲素Triton X-100微乳剂,并测定了其光热稳定性及对植物病原菌的抑制活性。结果表明:在0-54℃、照度低于3600 1x时,该微乳剂的光热稳定性良好;在照度为7200 1x时,50mg/L的HA-TX-100微乳剂对番茄灰霉病菌Botrytis cinerea、松针褐斑病菌Lecanosticta acicola、油菜菌核病菌Sclerotinia sclerotiorum和小麦赤霉病菌Fusarium graminearum的抑制率均超过50%,其中对油菜菌核病菌的抑制率最高,达77.60%;1000mg/L的HA-TX-100微乳剂对活体番茄接种番茄灰霉病菌的保护作用与治疗作用抑制率分别为72.05%和64.73%,均优于相同条件下市售50%多菌灵可湿性粉剂的作用。研究结果表明,竹红菌甲素具有开发为光活化农药的潜力。
Hypocrellin A(HA) is easy to dissolve in organic solvent,but difficult to dissolve in water, which has been a bottleneck problem on the application of HA. Thus a new water-soluble fungicide HA- TX-100 micro-emulsion(ME) was developed by embedding HA in Triton X-100. The light & thermal stability and the antimicrobial activities of HA-TX-100 ME were investigated under laboratory conditions. The results showed that HA-TX-100 ME had good stability when the temperature was 0 -54 ℃ and the light intensity was below 3 600 1x. Moreover, when the light intensity was 7 200 1x, 50 mg/L HA-TX-100 ME showed high antimicrobial activity against Botrytis cinerea, Lecanosticta acicola, Sclerotinia sclerotiorum and Fusarium graminearum, and the inhibition rates were all over 50%. The fungicide had the best inhibitory effect on S. sclerotiorum with inhibition rate reached 77.60%. The protective and therapeutic inhibition rates of 1 000 mg/L HA-TX-100 ME against B.cinerea were 72. 05% and 64. 73% respectively, which were significantly higher than that of carbendazim wetable powder under the same condition. The results of present study showed that HA had the potential to be developed as photoactive pesticide.
出处
《农药学学报》
CAS
CSCD
北大核心
2013年第5期587-592,共6页
Chinese Journal of Pesticide Science
基金
浙江省自然科学基金项目(LY12C14005)
国家级大学生创新创业训练计划项目(201310341003)
浙江省大学生科技创新活动计划(新苗人才计划)项目(2013R412004)
浙江农林大学创新训练计划项目(20120201)