STAT3在骨肉瘤中的表达及其与肿瘤微血管密度的关系

The expression of signal transducers 3 and activators of transcription 3 in osteosarcoma and its relationship with tumor microvascular density

目的:探讨信号转导和转录活化因子3(STAT3)在骨肉瘤组织中的表达及临床意义,并分析其与肿瘤微血管密度(MVD)的关系。方法:应用免疫组织化学法检测STAT3的表达,并对47例骨肉瘤患者的主要临床资料、肿瘤分期及临床相关参数进行比较,同时计算骨肉瘤组织的MVD,分析骨肉瘤组织中STAT3表达与MVD的相关性。结果:STAT3主要在细胞质中表达,在骨肉瘤组织中的表达均显著高于正常骨组织(P<0.01);在骨肉瘤Ⅱa期、Ⅱb期、Ⅲ期的STAT3表达均显著高于Ⅰ期(P<0.01),Ⅲ期的STAT3表达均显著高于Ⅱa期和Ⅱb期(P<0.01)。软组织浸润组的STAT3表达显著高于非浸润组(P<0.01)。骨肉瘤组织中STAT3表达与MVD呈正相关关系(P<0.05)。结论:STAT3的表达与骨肉瘤的肿瘤分期有关,而且与肿瘤有无软组织浸润密切相关;STAT3在骨肉瘤中的表达与MVD呈正相关关系。提示STAT3的高表达可能参与了骨肉瘤的发生、发展,尤其是与骨肉瘤的血管形成密切相关。

Objective： To explore the expression of signal transducer and activator of transcription 3 （ STAT3 ） in osteosarcoma and its relationship with tumor microvascular density（MVD）. Methods：The STAT3 expression of 47 cases with osteosarcoma were detected by immunohistochemistry. The clinical data, pathological classification and clinical index of 47 cases were compared. MVD of osteosarcoma was counted. The correlation between the expression of STAT3 and MVD was analyzed. Results：The exprssion of STAT3 was mainly in the cytoplasm. The expression levels of STAT3 in osteosarcoma were significantly higher than that in normal bone tissue（ P 〈 0.01 ）. The expression levels of STAT3 in osteosarcoma with stage ]I a, ]Ib and m were significantly higher than those in stage [ （ P 〈 0.01 ）, the expression of stage m was significantly higher than stage II a and ]1 b（ P 〈 0.01 ）. The expression levels of STAT3 in invasive soft tissue were significantly higher than that in no-invasive soft tissue（ P 〈 0.01 ）. The expression of STAT3 in osteosarcoma was positively correlated with MVD （P 〈 0.05 ）. Conclusions：The expression of STAT3 in osteosarcoma is correlated with tumor clinical stage and soft tissue invasion, and its expression is positively correlated with MVD. The high expression of STAT3 may play an important role in the development of osteosarcoma, and which is closely correlated to the angiogenesis of osteosarcoma.