鸭病毒性肝炎病毒抗原检测的研究

Detection of Virus Antigen of Duck Viral Hepatitis

将病鸭的肝脏匀浆冻融后点于硝酸纤维素膜 ,建立了检测鸭肝炎病毒抗原的 Dot-ELISA法 ,对采集的 52份肝脏检测表明 ,鸭肝炎病毒的检测率为 73% ( 38/52 )。同时对肝脏进行微生物学检查 ,鸭疫里氏杆菌的检测率为 52 % ( 2 7/ 52 )。两种病原的混合感染率为 2 7% ( 1 4/ 52 )

Dot-ELISA was established for detection of duck viral hepatitis antigen from 52 attacked ducks liver samples.The results showed that the positive rate was 73%(38/52).Meanwhile,we also carried on microbiological test of the samples,27(52%)strains Riemerella anatipestifer were isolated from 52.The rate of infected with two pathogen was 27%(14/32).All this provided detection methods and theory basis for the practices.