mTOR抑制剂Rapamycin对细胞株786-O增殖和凋亡的影响

The effect of mTOR inhibitor Rapamycin in the proliferation and apoptosis of cell lines 786-O

目的观察mTOR抑制剂Rapamycin对细胞株786-O增殖和凋亡的影响。方法采用四甲基偶氮唑盐(MTT)检测细胞的增值能力,采用Hochest染色检测细胞凋亡情况,观察细胞增殖、凋亡情况。结果 Rapamycin可显著降低细胞的增殖能力,呈时间和浓度依赖性。在较高浓度下显著引起786-O细胞出现凋亡小体,并表现为剂量依赖关系。结论 mTOR抑制剂Rapamycin具有阻滞细胞周期和诱导凋亡的作用。

Objective To analysis the efficacy of mTOR inhibitor Rapamycin in the proliferation and apoptosis of cell lines 786-0. Methods The cell proliferation ability was detected by methyl thiazolyl tetrazolium salt. The cell apoptosis was tested by hochest staining. Observed the cell proliferation and cell apoptosis. Results Rapamycin can effectively reduce the cell proliferation,in a time and close - dependent manner. Rapamycin at higher concentrations can lead to apoptotic bodies. Conclusion mTOR inhibitor Rapamycin has an function of cell cycle arrest and induction of apoptosis.