白细胞介素2(^(125)Ser)在昆虫细胞中的高效表达

High Expression of Human^(125)Ser IL-2 in Insect Cells

目的 高效表达白细胞介素 2 (12 5Ser)。方法 采用新型Bac -to -Bac杆状病毒表达系统 ,以PCR技术扩增了人白细胞介素 2 (12 5Ser)的基因片段 ,将其克隆到转座载体 ,经转座作用将白细胞介素 2 (12 5Ser)基因插入杆状病毒穿梭载体 ,在昆虫细胞中表达。结果 通过免疫印迹实验及CTLL - 2细胞 /MTT法检测 ,表达产物具有人白细胞介素 2的免疫学及生物学活性。经SDS -PAGE电泳 ,薄层扫描分折 ,表达量达到了 2 3.47%。结论 白细胞介素 2 (12 5Ser)在昆虫细胞中获得了高效表达。

Objective For highly expression of human 125 Ser IL 2.Methods Human 125 Ser IL 2 gene was amplified by PCR,then cloned into transposition vector and expressed using a novel Bac to Bac baculovirus expression system.The immunological and biological activities of expressed protein were detected by Western blot and CTLL 2/MTT method respectively.Results The expressed protein showed the immunological and biological activities of human IL 2.SDS PAGE proved that the expression level of the protein reached 23.47% .Conclusion Human 125 Ser IL 2 is highly expressed in insect cells.