摘要
在两种培养体系下对牛胚胎早期发育进行了比较研究。把屠宰场收集的牛卵体外培养至成熟 ,然后进行体外受精。将受精卵置于下列两种培养体系中进行体外培养 :①Buffaloratliver细胞与改进的TCM199培养液共同培养系统 (BRL/mTCM199) ;②半确定培养液两步培养系统 [无血清的金黄地鼠培养液与改进的TCM 199培养液 (HECM - 6 /mTCM 199) ]。结果表明 :后者的囊胚率 (5 0 % )明显高于前者的囊胚率 (4 1% ,P<0 0 1)。表明牛胚胎早期分裂可在无蛋白培养条件下进行 ,并且在此培养条件下有良好的后续发育能力。这可能与两步培养体系中葡萄糖和氨基酸浓度不同有关。
Cumulus oocyte complexs (COC) were recovered from abattoir cow ovaries collected from slaughterhouse. Then,They were matured and fertilized in TCM199 with 10% BCS. Later on,the one cell embryos were cultured in two culture systems:①modified TCM199 over a buffalo rat liver (BRL) monolayer co culture;②Two stage semi defined culture system [hamster embryo culture medium with 11 amino acids (HECM 6) followed by tissue culture medium 199+10% bovine calf serum]. The results showed that HECM 6/mTCM199 system could improved embryo development to the morula or blastocyst stage higher (50%) than that in BRL/mTCM199 system (41%, P <0 01). This results suggested that HECM 6/mTCM199 two stage semi defined culture system could provide a significant benefit for the early bovine embryo development,which might be related with glucose concentration and amino acids components in the medium.
基金
云南省自然科学基金!(96C0 0 6Z)
云南省省院省校合作项目!(YK980 0 4)资助
